WBC 307 Review

the following indicates ____:

• CD3 +

• CD4 +

t helper cells

the following indicates ____:

• CD3 +

• CD8 +

T cytotoxic cells

the following indicates ____:

• CD19 +

• CD20 +

• CD3 -

B cells

the following indicates ____:

• CD11c +

• CD123 +

dendritic cells

the following indicates ____:

• CD56 +

• CD16 +

• CD3 -

NK cells

the following indicates ____:

• CD20 -

• CD25 -

• CD34 +

HSCs

the following indicates ____:

• CD14 +

• CD33 +

macrophages/monocytes

the following indicates ____:

• CD66b +

granulocyte

the following indicates ____:

• CD41 +

• CD61 +

• CD62 +

platelets

which population is negative for both property 1 and 2?

A

which population is strongly positive for property 2 but negative for property 1?

C

which population is positive for both property 1 and 2?

D

which population is strongly positive for property 1 but negative for property 2?

B

the more shift to the ____, the more intense the fluorescence signal (higher expression of the antigen/”positive”)

right

WB or BM samples for flow cytometry should be used within the first ____ of collection.

4 hours

which stain are maturing granulocytes positive for?

MPO

which stain differentiates AML from ALL?

SBB

which stain stains cellular lipids?

SBB

which stain are granulocytes from myeloblast through maturation series positive for (*the more mature the cell, the stronger the reaction)?

SBB

naphtol AS-D chloroacetate esterase (NASDA) is a specific esterase that is positive in what cells?

granulocytes

naphtol AS-D chloroacetate esterase (NASDA) is a specific esterase that is negative in what cells?

monocytic cells

alpha-naphthyl acetate and alpha-naphthyl butyrate (nonspecific esterases) are strongly positive for what cells?

monocytic cells

what reactions are used to differentiate myeloblasts and neutrophilic granulocytes from cells of monocytic origin?

esterase

what stain stains carbohydrates?

periodic acid schiff

which stain is positive for the following:

• megakaryocytes

• PLTs

• leukemic erythroblasts

periodic acid schiff

immunostaining for FVIII and vWF in the alpha granules will be positive for ____ leukemia.

megakaryocytic

what stain is used for T-cell ALL and HCL.

acid phosphatase

what stain is specific for HCL?

TRAP

what condition is associated with the following stain results:

• MPO (-)

• SBB (-)

• NASDA/specific (-)

• ANBE/nonspecific butyrate (-/+; focal)

• ANAE/nonspecific acetate (-/+; focal)

ALL

what condition is associated with the following stain results:

• MPO (+)

• SBB (+)

• NASDA/specific (+)

• ANBE/nonspecific butyrate (-)

• ANAE/nonspecific acetate (-)

AML

what condition is associated with the following stain results:

• MPO (+)

• SBB (+)

• NASDA/specific (+)

• ANBE/nonspecific butyrate (+; diffuse)

• ANAE/nonspecific acetate (+; diffuse)

AMML (M4)

what condition is associated with the following stain results:

• MPO (-)

• SBB (-/+)

• NASDA/specific (-)

• ANBE/nonspecific butyrate (+; diffuse)

• ANAE/nonspecific acetate (+; diffuse)

AMoL (M5)

what condition is associated with the following stain results:

• MPO (-)

• SBB (-)

• NASDA/specific (-)

• ANBE/nonspecific butyrate (-)

• ANAE/nonspecific acetate (+; localized)

megakaryocytic leukemia

alpha-naphthyl acetate/butyrate esterase is ____ in granulocytes and lymphoid cells.

negative

the reaction for alpha naphthyl acetate/butyrate esterase can be inhibited by ____.

NaF