RP1

Variables that could affect the rate of an enzyme-controlled reaction

Enzyme concentration/ volume
Substrate concentration/ volume
Temperature of solution
pH of solution
Inhibitor concentration

Describe how pH can be controlled.

Use a buffer solution

Describe how temperature can be controlled.

Use a hot water bath and monitor using a thermometer at regular intervals.

Why were enzyme and substrate solutions left in water baths for 10 minutes before mixing?

so solutions equilibrate/ reach the temperature of the water bath.

What was the enzyme we used in our practical?

trypsin

What was the substrate we used in our practical?

milk powder