Combined Study Review-(HPLC, GC-FID, GC-MS, & FAA)

A collection of flashcards reviewing key concepts and procedures related to HPLC, GC-FID, GC-MS, and FAA techniques.

What does HPLC stand for?

High-Performance Liquid Chromatography

What is the purpose of the C18 column in HPLC?

It retains nonpolar analytes longer.

What is the mobile phase used in HPLC?

Water + Methanol.

What does the output of HPLC indicate regarding peak area?

Peak area is proportional to concentration.

What type of injector is used in HPLC?

Split Injector controls how much sample enters the column.

What does FID stand for in GC-FID?

Flame Ionization Detector.

What is the primary function of the FID detector?

It detects ionized carbon species.

In GC-FID, what does the output peak area indicate?

Peak area is proportional to the amount of volatile organics.

What ionization method is used in GC-MS?

Electron Ionization (EI) at 70 eV.

What does TIC stand for in the context of GC-MS?

Total Ion Chromatogram.

What is the function of the HCl lamp in FAA?

It matches specific metal wavelengths.

What is the output measurement related to in FAA?

Absorbance is proportional to metal concentration in ppm.

What are the different separation bases of HPLC, GC-FID, GC-MS, and FAA?

HPLC relies on polarity, GC-FID and GC-MS rely on volatility, and FAA involves atomization.

What is the required injection volume for HPLC?

5–20 μL.

What should you do if there are no peaks in your HPLC results?

Check λmax and sample preparation.

What is the critical adjustment needed for the burner in FAA?

Burner height and fuel/air ratio.

What is the significance of the match score greater than 900 in GC-MS?

It indicates strong confidence in the identification.

How do you correct matrix interference in FAA?

Use standard addition.

What does broad or tailing peaks in HPLC suggest, and how can it be fixed?

It suggests clean column or flow adjustments.

How can you establish a calibration curve in HPLC?

By plotting area vs concentration.