Metabolism
All of the chemical reactions in an organism
Metabolic Pathways
Series of chemical reactions that either build complex molecules or break down complex molecules
Catabolic Pathways
Pathways that release energy by breaking down complex molecules into simpler compounds
Anabolic Pathways
Pathways that consume energy to build complicated molecules from simpler compounds
Thermodynamics
the study of energy transformations in matter
1st Law of Thermodynamics
Energy cannot be created or destroyed, only transformed from one form to another.
2nd Law of Thermodynamics
Energy transformation increases the entropy of the universe. During energy transfers or transformations some energy is unusable and often lost as heat.
Exergonic Reactions
Reactions that release energy
Endergonic Reactions
Reactions that absorb energy
Mechanical
movement
Transport
pumping substances across membranes against spontaneous movement
Chemical
Synthesis of molecules
Adenosine Triphosphate
molecules that organisms use as a source of energy to perform work
Phosphorylation
the released phosphate moves to another molecule to give energy
Enzymes
macromolecules that catalyze reactions, lowering the activation energy
Induced Fit
Enzyme will change the shape of their active site to allow the substrate to bind better
Optimal Conditions
The conditions (temperature and pH) that allow enzymes to function optimally
Cofactors
non-protein molecules that assist in enzyme function
Holoenzyme
an enzyme with the cofactor attached
Coenzymes
Organic cofactors
Enzyme Inhibitors
Reduce the activity of specific enzyme
Competitive Inhibitors
Reduce enzyme activity by blocking substrates from binding to the active site.
Noncompetitive Inhibitors
Bind to an area other than the active site which changes the shape of the active site prevents substrates from binding
Allosteric Regulations
molecules bind (noncovalent allosteric interactions) to an allosteric site which changes the shape and function of the active site
Allosteric Inhibitor
Substrate binds to allosteric site and stabilizes the enzyme shape so that the active sites are closed
Cooperativity
Substrate binds to one active site (on an enzyme with more than one active site) which stabilizes the active form
Feedback Inhibition
Sometimes the end product of a metabolic pathway can act as the inhibitor to an early enzyme in the same pathway
Autotrophs
Organisms that produce their own food from simple substances in their surroundings
Heterotrophs
Organisms unable to make their own food so they live off of other organisms
Cyanobacteria
early prokaryotes capable of photosynthesis
Photosynthesis Formula
6CO2+6H2O+Light Energy —→ C6H12O6+6O2
Redox Reaction
Reaction involving complete or partial transfer of one or more electrons from one reactant to another
Oxidation
Loss of electrons
Reduction
Gain of electrons
Light
Electromagnetic energy
Wavelength
the distance from the crest of one wave to the crest of the next
Photorespiration
On very hot days plants close their stomata to stop water loss. Causes less Carbon Dioxide to be present and more oxygen. Rubisco binds to oxygen and uses ATP
C4 Plants
Spatial separation of steps. Stomata partially close to conserve water. Mesophyll cells fix Carbon dioxide into a 4-C molecule. Transferred to bundle sheath cells.
CAM Plants
Open stomata at night and close during the day. Carbon dioxide is incorporated into organic acids and stored in vacuoles. During the day, light reactions and Carbon dioxide is released from the organic acids and incorporated into the Calvin Cycle.
Electron Transport Chain
a sequence of membrane proteins that shuttle electrons down a series of redox reactions
Glycolysis
Starting point of cellular respiration
Occurs in the cytosol
Splits glucose (6C) into pyruvates (3C)
Energy Investment Stage
the cell uses ATP to phosphorylate compounds
Energy Payoff Stage
Energy is produced by substrate level phosphorylation.
Net energy yield per one glucose: 2 ATP @ NADH
Citric Acid Cycle
Also known as the Krebs cycle
Occurs in the mitochondrial matrix
Turns acetyl CoA into Citrate. Releases CO2 ATP synthesized; electrons transferred to NADH and FADH2
ATP Synthase
The enzyme that makes ATP from ADP+
1. Uses energy from the H+ gradient across the membrane
2. H+ ions flow down their gradient through ATP synthase.
Anaerobic Respiration
Generates ATP using an ETC in absence of oxygen. Takes place in prokaryotic organisms that live in environments with no oxygen.
Fermentation
Generates ATP without an ETC. Extension of glycolysis. Recycless NAD+
Alcohol Fermentation
Pyruvate is converted to ethanol
Lactic Acid Fermentation
Pyruvate is reduced directly by NADH to form lactate