Endocrinology and Hormone Assays

What are standards

Solutions with known concentrations used to create a standard curve

What does NSB stand for

Non specific binding

What is not in an NSB

antibody

What does the NSB wells tells us

the background

What is binding zero b0

0 hormone in the sample (control)

What kind of well is this

NSB

What well is this

B0

Steps for EIA

1) Collect samples

2) Make standards for assay

3) Add buffers/samples/antibodies…

4) Draw standard curve and calculate sample concentrations

you can get output values for…?

Radioactivity (RIA) or optical density (EIA)

What hormone does a pregnancy test detect

hCG

Ovulation tests test for high levels of

LH

Half lives of FSH LH and hCG ranked from shortest to longest

LH<FSH<hCG

FSH, LH, and hCG share a common ___ subunit, but different ___ subunit

alpha, beta