Phenol Red Broth Test

These flashcards cover the key terms and concepts related to the Phenol Red Broth test used for lactose fermentation.

Phenol Red Broth

A differential medium used to test for lactose fermentation.

Lactose Fermentation

The metabolic property being tested in Phenol Red Broth.

Substrate in Phenol Red Broth

Lactose and Peptone are the substrates contained in the media.

Indicator for Lactose Fermentation

Phenol Red is the indicator used to detect lactose fermentation.

Durham Tube

An indicator for CO2 production in the Phenol Red Broth test.

Yellow Phenol Red

Indicates fermentation of lactose.

Pink Phenol Red

Indicates degradation of peptone.

Positive result appearance

Lactose fermentation results in yellow coloration. CO2 production indicated by an air bubble in the Durham tube.

Negative result appearance

No coloration change and no air bubble in the Durham tube.

Reagents for Phenol Red Broth Test

No reagents are required for this test.