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What is optimum temperature?
The temperature at which an enzyme works best.
What is optimum pH?
The pH at which an enzyme works best.
What happens if enzymes get too hot or the pH is wrong?
They denature (lose shape) and stop working.
What happens if substrate concentration is too low?
The reaction is slow.
What happens if substrate concentration is too high?
The enzyme becomes saturated, so the rate stops increasing.