Kaarten: Pph6 blood analysis | Quizlet

Blood parameters: hemaglobin level, hematocrit and RBC count

Gives info about O2 binding capacity

Blood parameters: ions and nutrient concentrations

excitability of cells and metabolic function

Blood parameters: levels of protein

osmotic value of blood an clotting-capacity (fibrinogen), defence mechanism of blood (globilins) and the capacity of capillary exchange (albumin)

Plasma

- Plasma, so lots of Protein
- Contains anticogulants and clotting factors
- Cell-free

serum

plasma without clotting factors
Does NOT have anticoagulants

Characteristics anemia

Normal hematocrit but lower hemoglobin

Characteristics high altitude

High hematocrit (epo) and higher hemoglobin

What happens to albumin when malnutritioned?

Albumin concentration is lower, absorption is lower than filtration and fluid accumulates in the belly.

Adding fibrinogen to serum

Serum becomes solid since it does not contain clotting factors

Can free amino acids disrupt the determination of the protein concentration

No, because free amino acids have no peptide bond

Precipitation

Rendering insoluble due to, for example temperature reduction, salt or centrifugation

Denaturing

Protein falling apart, thus breaking (i.e by boiling or pH).(stomach). you have a loss of structure and function

Solidification

Natural transition from liquid to solid. molecules become one, sit together an form a complex, for example the fibrin complex

Why does fibrogen isolation include washing and why does this require ice water

lowering the solubility of fibrogen by lower temparature. wash away contaminants, including the anticoagulant in plasma, namely heparin.

What is the effect of alcohol during the isolation

Organic solvents reduce the solubility of proteins, the action of salts become better so that firbrinogen precipitates more easily