DNA Sequencing and BLAST Overview

These flashcards cover key concepts from DNA sequencing and BLAST search results.

The numerical scores assigned by BLAST are measured in __.

bits

An E-value of _ or less represents an acceptable match.

3

The scientific name of the bacterium sequenced is __.

Bartonella Henselae

Homology refers to __ from new sequence bears a significant degree.

similarities

BLAST stands for __.

Basic Local Alignment Search Tool

The purpose of laser beams in DNA sequencing is to read based on __.

the fluorescence of nucleotides

The purpose of the second PCR is to produce many copies of __.

variable lengths

The final PCR product contains a mix of DNA pieces of __ length.

variable

The purpose of gel electrophoresis is to separate molecules based on __.

differences in size

DNA molecules move towards the __ end due to applied electric current.

positive