Microbiology Lab Exercise 9

Fermentation is a form of energy metabolism where the final electron acceptor is

organic

Anaerobic respiration is a form of energy metabolism where the final electron acceptor is

inorganic

Aerobic respiration is a form of energy metabolism where the final electron acceptor is

oxygen

Which molecule is hydrolyzed by proteases?

casein

Which molecule is hydrolyzed by amylase?

starch

Which molecule is hydrolyzed by lipases?

fat

Which molecule is deaminated into phenylpyruvic acid and ammonia?

phenylalanine

Which molecule is degraded in indole, ammonia and pyruvic acid?

tryptophan

Which molecule splits in carbon dioxide and ammonia when it is hydrolyzed?

urea

The mixed acid fermentation test uses which reagent to indicate a positive reaction?

methyl red

Which enzyme is tested for with hydrogen peroxide?

catalase

The 2,3 butanediol fermentation test uses which reagent to indicate a positive reaction?

barritt

Which enzyme is tested for in an oxidation test?

oxidase

Name one of the tests that must be completed under anaerobic conditions in the API20E

LDC

Name one of the tests that examines carbon utilization in the API20E

MAN

Name one of the tests that examines nitrogen utilization in the API20E

URE

Name one of the tests that examines protein utilization in the API20E

IND

For the starch hydrolysis test, the image shows a ___ response.

positive

For the starch hydrolysis test, the image shows a ___ response.

negative

For the casein hydrolysis test, the image shows a ___ response.

positive

For the casein hydrolysis test, the image shows a ___ response.

negative

For the fat hydrolysis test, the image shows a ___ response.

negative

For the fat hydrolysis test, the image shows a ___ response.

positive

For the tryptophan hydrolysis test, the image shows a ___ response.

positive

For the tryptophan hydrolysis test, the image shows a ___ response.

negative

For the urea hydrolysis test, the image shows a ___ response.

negative

For the urea hydrolysis test, the image shows a ___ response.

positive

For the phenylalanine deamination test, the image shows a ___ response.

negative

For the phenylalanine deamination test, the image shows a ___ response.

positive

For the catalase test, the image shows a ____ response.

positive

For the catalase test, the image shows a ____ response.

negative

For the mixed acid fermentation test, the image shows a ____ response.

negative

For the mixed acid fermentation test, the image shows a ____ response.

positive

For the 2,3 butanediol fermentation test, the image shows a ____ response.

negative

For the 2,3 butanediol fermentation test, the image shows a ____ response.

positive

Which image of the API20E arginine dihydrolase (ADH) test is positive?

Which image of the API20E citrate utilization (CIT) test is positive?

Which image of the API20E glucose utilization (GLU) test is negative?

Which image of the API20E typtophane deaminase (TDA) test is negative?

Which image of the API20E beta-galactosidase (ONPG) test is positive?