WBC ID and facts

myeloblast picture

myeloblast

immature bone marrow cell that gives rise to granulocytes

promyelocyte picture

Promyelocyte

Appearance of primary/nonspecific granules

myelocyte picture

Myelocyte

immature cell that comes from a myeloblast in the red marrow and develops into either a neutrophil, eosinophil, or basophil

Metamyelocyte picture

Metamyelocyte

transitional cell intermediate in development between a myelocyte and a mature granular leukocyte

what is the order of granulocyte development?

• 1st Myeloblast

• 2nd Promyelocyte

• 3rd Myelocyte

• 4th Metamyelocyte

• End of proliferative phase

Segmented neutrophil picture

band neutrophil picture

segmented neutrophil characteristics

nucleus: pinched into segments connected with a fine filament, 3-5 lobes distinct lobes, chromatin coarse densely packed,

cytoplasm: abundant, pink

granules: secondary granules fine and specific

10-16 micro meter

most abundant

Band neutrophil characteristics

elongated/curved nucleus

(associated with C, S, or J shape

pink cytoplasm

chromatin is clumped

10-16 micro meter

eosinophil picture

Eosinophil characteristics

nucleus is bilobed,

secondary cytoplasmic granules stain red to orange

cytoplasm is irregular and pink

chromatin is coarse clumped

12-17 micrometers

primary granules rare

bright and colorful

basophil picture

Basophil characteristics

-nucleus 2 lobed no visible chromatin

-cytoplasmic granules stain dark purple

cytoplasm is lavender to colorless

cytoplasm predominates N:C ratio

very dark

steps of monocyte maturation

. Monoblast (same description as myeloblast)

2. Promonocyte◦Size: 12 to 20 micrometers◦Irregular and indented chromatin; gray-blue cytoplasm

3. Monocyte◦Chromatin is loosely weaved, lacy, open, and thin;cytoplasm may have numerous vacuoles

Monocyte characteristics

-Largest WBC

-Have "U"-shaped nucleus

chromatin is lace like

can have vacuoles

color is blue grey for cytoplasm can be irregular shaped

granuoles fine dispered and abundant

monocyte picture

Neutrophil function

phagocytize bacteria

eosinophil function

Kill parasitic worms and is important in chronic allergeries

Smaller round granules rich in acid phosphatase◦Larger containing acid phosphatase

Basophil function

release histamines + herperine (inflammatory response)

acute allergies like anaphylaxis

Contain heparin and histamine

Monocytes/Macrophages function

Ingest and kill microorganisms

Inhibit the growth of intracellular microorganisms

Removal of senescent RBCs, dead cells, and debris

Maintain storage for iron

Synthesize proteins

what cells are myeloid

neutrophils, monocytes, eosinophils, and basophils

what cells are granulocytes

neutrophils, eosinophils, basophils

what is the lymphatic cell population?

T cells 60%-80% B cells 10-20% NK cells <10%

T lymphocyte function

Cellular immune response; destroys virally infected cells and cancer cells

mature in thymus

60-80%

B lymphocyte function

antibody production

mature in BW

10-20%

natural killer cell function

Non-selectively attacks non-self cells, especially body cells that have undergone mutation and become malignant, also attacks grafts and transplanted organs (CELL-MEDIATED)

less than 10%

lymphocyte primary maturation sites

BM and thymus

lymphocyte secodnary maturation sites

Spleen, lymph nodes, Peyer's patches of GI tract, and tonsils

lymphocyte maturation order

Lymphoblast

Prolymphocyte

Lymphocyte

lymphocyte picture

lymphocyte characteristics

small amount of cytoplasm

nucleus is very dark

typically blue

chromatin is dense and clumped

relative percentage lymphocytes at birth

31%

relative percentage lymphocytes at 6 months

61%

relative percentage lymphocytes at 10 yrs

38%

relative percentage lymphocytes at 21 and up

34%

relative percentage of neutrophils

50-70%

relative percentage of lymphocyte

18-42%

relative percentage of monocyte

2-11%

relative percentage of basophil

0-2%

relative percentage of eosinophils

1-3%