Quiz 5 Microlab
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20 Terms
Purpose of gelatinase test
To determine if a microorganism produces the gelatinase enzyme which breaks down into amino acids
Enzyme in gelatinase test
Gelatinase
Substrate in gelatinase test
Gelatin
Purpose of spirt blue test (Lipid test)
To determine if a microorganism produces lipase to break down lipids (fats)
Enzyme in spirit blue (lipid) test
Lipase
Substrate in spirit blue (lipid) test
Lipid
Purpose of casein hydrolysis (casease) test
To determine if a microorganism can produce casease (a protease/protein) that breaks down casein into small peptides/amino acids
Enzyme in casein hydrolysis test
Caseinase (protease)
Substrate in casein hydrolysis test
Casein (milk protein)
Purpose of coagulase test
To determine whether bacteria produce the enzyme coagulase which causes plasma to clot
Enzyme in coagulase test
Coagulase
Substrate in coagulase test
Fibrinogen (in the plasma)
which side is a positive casein hydrolysis test
right side (b, cereus)
which side is a negative casein hydrolysis test
left side (e. coli)
which is a positive gelatinase test
top tube (s. aureus)
which is a negative gelatinase test
bottom tube (s. epi)
what are the positive results of a coagulase test?
clot formation
what bacteria is positive in the coagulase test?
S. aureus
what does a positive spirit blue test look like?
clear halo around streak
what bacteria is positive in spirit blue test?
s. aureus
