Lecture 2 - Ion-Exchange Chromatography

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5 Terms

1
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The column contains

resin with either a net positive or negative charge and is used to bind to an analyte with the opposite charge.

2
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What happens to analytes with the opposite charge?

Bind

The bound analyte is removed or eluted by changing the pH of the column.

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What happens to analytes with the same charge?

Move through the column

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Ion exchange chromatography separates based on

charge

5
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Clinical Application

(a). Remove interfering substances from a sample

(b). Separate sample with various amino acids