METHODS OF DIRECT OBSERVATION

EXTERIORIZATION & TRANSILLUMINATION

ex: take organ out of rat and observe under the light

TRANSPARENT CHAMBER

ex: taking a piece of tissue or organ and placing in the middle of two glass sheets and observed

what can you do with the transparent chamber method?

• observe the growth of capillaries and nerves

• observe the emigration of leukocytes

• observe the growth of adipose tissue

what can you do with the exteriorization and transilumination method?

• observe blood circulation in the organs

• observe ovulation in rats

• observe secretory properties in pancreatic cells

which methods gets extended?

transparent chamber

explained: use the cornea of a monkey and input ovarian cells into it and the the stages of ovarian cells.

cell and organ culture

ex: cells that are cultured in a laboratory and are growed in such environment

what can you do with the cell and organ culture method?

• observe the growth of fibroblast

• observe the growth of primordium

mechanincal micromanipulation

ex: the use of a mechanical tool (like pipettes/needles), that places things into a single cell or out of a cell

what can you do with the mechanical micromanipulation method?

• take the nucleus out of cell and see if cell survives (we know it dies)

radiation probe

ex: use of radiation to produce macromolecules (proteins)

what can you do with radiation probe method?

• observe amino acids form into proteins

vital and supravital staining

• cells are incubated into specific medium

what can you do with the vital and supra vital staining?

• observe certain colors specific to the nucleus and mitochondira