session 10 Protein Structure, analysis and Function

Protein Structure

Functionality depends on three-dimensional arrangement.

Fluorescent Proteins

Proteins that emit light upon excitation.

Located in the geometric centre of the molecule.•

Autocatalytic reaction in presence of O2.

Genetic coding of color and fluorescence

GFP

Green fluorescent protein used for tracking.

Ser-Tyr-Gly

Tripeptide forming the core of fluorescent proteins.

Applications of GFP and derivatives

fusion protein tracking

Ion Affinity Chromatography

Technique for purifying proteins using charged ions.

IMAC

Immobilized Metal Ion Affinity Chromatography for protein purification.

GST Tagging

Method for protein purification using glutathione S-transferase.

Immunoprecipitation

Technique to isolate proteins using antibodies.

Elution Step

Process of releasing bound proteins from chromatography.

Bacterial Expression

Using bacteria to produce recombinant proteins.

Fusion Protein Tracking

Monitoring protein interactions using fluorescent tags.

asFP595

Red fluorescent protein with 595nm emission.

asFP499

Fluorescent protein with 499nm emission.

Quaternary Structure

Complex formed by multiple protein subunits.

Gel Filtration Chromatography

Technique separating proteins by size.

Optical Detector

Device measuring light intensity in chromatography.

Autocatalytic Reaction

Self-catalyzing reaction requiring oxygen for GFP.

Recombinant Protein Production

Creating proteins through genetic engineering methods.

Polyhistidine Binding

Interaction of histidine residues with metal ions.