micro1 Microscopy & Molecular Methods
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These flashcards cover key concepts from Chapter 5 on Microscopy and Molecular Methods.
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14 Terms
What is the sequence of steps in Gram staining?
Crystal violet → Iodine → Decolorizer → Safranin
What color indicates a Gram-positive bacteria after staining?
Purple
What color indicates a Gram-negative bacteria after staining?
Pink
Which stain is used for identifying Mycobacterium due to mycolic acids?
Acid-fast stain
What type of microscopy is typically used for routine examination?
Brightfield microscopy
What microscopy type is best for visualizing spirochetes?
Darkfield microscopy
Which type of microscopy allows for the observation of live organisms?
Phase contrast microscopy
What type of microscopy uses UV light and fluorochromes?
Fluorescent microscopy
What special preparation is used for detecting fungi?
KOH preparation
What special preparation is used for visualizing capsules?
Nigrosin preparation
What special preparation is used for observing parasites?
Iodine mount
What is the function of nucleic acid probes in molecular methods?
To detect DNA sequences
What does PCR stand for and what is its purpose?
Polymerase Chain Reaction; it amplifies DNA and is the most sensitive rapid method.
What does sequencing help determine?
The nucleotide order of DNA.
Note 
Flashcards (24)