BCHM 3050 Exam 4 (Beebe, Clemson) - Reactants/Enzymes/Products

Step 1 Glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

Glucose, Hexokinase, Glucose-6-phosphate

Step 2 Glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

Glucose-6-phosphate, phosphoglucose isomerase, Fructose-6-phosphate

Step 3 glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

Fructose-6-phosphate, PFK1, Fructose-1,6-Bisphosphate

Step 4 Glycolysis

Starting material: _________

Enzyme: ___________

Products: ___________ and __________

Fructose-1,6-Bisphosphate, aldolase, G3P Dihydroxyacetone phosphate

Step 5 Glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

Dihydroxyacetone phosphate, Triose phosphate isomerase, G3P

Step 6 glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

G3P, G3P dehydrogenase, 1,3-bisphosphoglycerate

Step 7 glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

1,3-bisphosphoglycerate, phosphoglycerate kinase, 3-phosphoglycerate

Step 8 glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

3-phosphoglycerate, phosphoglycerate mutase, 2-phosphoglycerate

Step 9 Glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

2-phosphoglycerate, enolase, Phosphoenolpyruvate

Step 10 Glycolysis

Starting material: _________

Enzyme: ___________

Product: ___________

Phosphoenolpyruvate, pyruvate kinase, pyruvate

Step 1 Gluconeogenesis

Starting material: _________

Enzyme: ___________

Product: ___________

Pyruvate, pyruvate carboxylase, oxaloacetate

Step 2 Gluconeogenesis

Starting material: _________

Enzyme: ___________

Product: ___________

Oxaloacetate, phosphoenolpyruvate carboxykinase, phosphoenolpyruvate

Step 3 Gluconeogenesis

Starting material: Fructose-1,6-bisphosphate

Enzyme: ___________

Product: ___________

Fructose bisphosphatase, fructose-6-phosphate

Step 4 Gluconeogenesis

Starting material: Fructose-6-phosphate

Enzyme: ___________

Product: ___________

phosphoglucose isomerase, glucose-6-phosphate

Step 5 Gluconeogenesis

Starting material: Glucose-6-phosphate

Enzyme: ___________

Product: ___________

Glucose-6-phosphatase, glucose

Pentose phosphate pathway step 1

Starting material: Glucose-6-phosphate

Enzyme: ___________

Product: ___________

Glucose-6-phosphate dehydrogenase, 6-phosphoglucono-d-lactone

Pentose phosphate pathway step 2

Starting material: 6-phosphoglucono-d-lactone

Enzyme: ___________

Product: ___________

6-phosphoglucono-lactonase, 6-phosphogluconate

Pentose phosphate pathway step 3

Starting material: 6-phosphogluconate

Enzyme: ___________

Product: ___________

6-phosphogluconate dehydrogenase, Ribulose-5-phosphate

Pentose phosphate pathway step 4

Starting material: Ribulose-5-phosphate

Enzyme: ___________

Product: ___________

Ribulose-5-phosphate isomerase, Ribose-5-phosphate

TCA Cycle Step 1

Starting material(s): ________ and ________

Enzyme(s): _________

Product(s): _______

Oxaloacetate, Acetyl-CoA, citrate synthase, citrate

TCA cycle step 2

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

Citrate, Aconitase, Isocitrate

TCA Cycle Step 3

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

Isocitrate, isocitrate dehydrogenase, a-ketoglutarate

TCA Cycle Step 4

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

a-ketoglutarate, a-ketoglutarate dehydrogenase, Succinyl-CoA

TCA Cycle Step 5

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

Succinyl-CoA, Succinyl-CoA synthetase, Succinate

TCA Cycle Step 6

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

Succinate, succinate dehydrogenase, Fumarate

TCA Cycle Step 7

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

Fumarate, fumarase, malate

TCA Cycle Step 8

Starting material(s): ________

Enzyme(s): _________

Product(s): _______

Malate, malate dehydrogenase, Oxaloacetate

Pyruvate Dehydrogenase Reaction One:

___________ ---> _____________-___________

Pyruvate, hydroxyethyl-TPP

Pyruvate Dehydrogenase Reaction Two:

___________ ---> ____________ + _____________

Hydroxyethyl, lipoamide, acetyl

Pyruvate Dehydrogenase Reaction Three:

___________ + ___________ ---> __________ + ___________ ___________

Lipamide, acetyl, acetyl-coA, reduced lipoamide

Pyruvate Dehydrogenase Reaction Four:

Lipoamide gets _____________

oxidized

Pyruvate Dehydrogenase Reaction Five:

FAD gets _____________

oxidized