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DNA Polymerase I
Removes RNA primer and replaces it with newly synthesized DNA
DNA Polymerase III
Main enzyme that adds nucleotides in the 5’-3’ direction
Helicase
Opens the DNA helix by breaking hydrogen bonds between the nitrogenous bases
Ligase
Seals the gaps between the Okazaki fragments to create one continuous DNA strand
Primase
Synthesizes RNA primers neded to start replication
Sliding Clamp
Helps to hold the DNA polymerase in place when nucleotides are being added
Topoisomerase
Helps relieve the strain on DNA when unwinding by causing breaks, and then resealing the DNA
Single-strand binding proteins (SSB)
Binds to single-stranded DNA to prevent DNA from rewinding back