Microbiology Laboratory Midterms

BASIC TOOLS IN MICROBIOLOGY - Microscope

Microscope

Optical tool used to observe microorganisms

Why is it called a Compound Microscope?

Intermediate object is projected by the objectives, then enlarged by the eyepiece

Parfocality

Viewed specimen stays in focus even when objectives are changed

Virtual Image

Final image formed; inverted

Magnification

How much larger object appears compared to actual size

How do you get the Total Magnification?

By multiplying the magnifying powers of the objective and eyepiece

Resolving Power

Measure of the clearness of the appearance of the specimen

Focal Length

Amount of distance required between the objective lens and the top of your object

Working Distance

Distance between the specimen and objective lens

Numerical Aperture

Number that indicates the angles over which light can enter the lens