Microscopy and Magnification

These flashcards cover key concepts from lectures on microscopy, magnification, and proper microscope usage.

Microscope

An instrument used to see objects that are too small for the naked eye.

Total Magnification

multiplying the ocular lens magnification by the objective lens magnification.

Inverted Image

An image that is turned upside down and reversed from left to right.

Lens Paper

Specialized paper used to clean the lenses of microscopes without scratching them.

3D Observation

see depth and dimension in an object using microscopy.

Ocular Lens

eyepiece lens of a microscope through which the specimen is viewed.

Objective Lens

lens or lenses on a microscope that are closest to the specimen and provide varying levels of magnification.

Specimen Orientation

The positioning and direction of a specimen being viewed under a microscope.

Focus Microscopy

The process of adjusting the focus of a microscope to see a clear image.

Living Cells

Cells that are currently functioning, as opposed to dead or preserved cells.

Types of microscopes

dissecting, compound, inverted

dissecting microscope

used for larger objects, 3D

compound microscopes

used for very small thing, has higher magnification

inverted microscope

for petri dish (liquid), and living cells

how should you hold a microscope

carry using 2 hands, 1 under base and 1 on arm

how to clean microscope lens

use only lens paper

how to focus microscope

start on lowest magnification, focus on object, increase magnification

clean/store microscope

first use lens paper to clean, then store scope w cord wrapped around base, and scanning objective in place, and stage fully lowered

total magnification

ocular lens x objective lens

orientation of specimen

images appear upside down & opposite

direction

To move must moves slide in opposite

direction to what is shown