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What does the Simmons Citrate Test for?
Citrate as sole source of carbon
(C’s match with Citrate)
What is a positive/negative citrate test?
Positive: Prussian blue color (ocean=good=positive)
Negative: Green color (swamp=bad=negative)
What does the Phenylalanine Deamination test for?
Presence of phenylalanase
What does phenylalanase do?
Breaks down phenylalanine into phenylpyruvic acid (PPA)
What reagent is added to Phenylalanine Deamination Test?
Several drops of 10% ferric chloride
What is a positive/negative phenylalanine deamination test?
Positive: Deep green color change (lala land=green=good= positive)
Negative: yellow (no color change)
What does SIM media deep test for?
Presence of H2S production
Presence of tryptophanase which can hydrolyze tryptophan into Indole and pyruvic acid
Motility