Microbio quiz 5 (unknown day 4)

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Last updated 1:16 AM on 4/7/26
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14 Terms

1
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What does the Simmons Citrate Test for?

  • Citrate as sole source of carbon

  • (C’s match with Citrate)

2
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What is a positive/negative citrate test?

  • Positive: Prussian blue color (ocean=good=positive)

  • Negative: Green color (swamp=bad=negative)

3
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What does the Phenylalanine Deamination test for?

  • Presence of phenylalanase

4
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What does phenylalanase do?

  • Breaks down phenylalanine into phenylpyruvic acid (PPA)

5
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What reagent is added to Phenylalanine Deamination Test?

  • Several drops of 10% ferric chloride

6
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What is a positive/negative phenylalanine deamination test?

  • Positive: Deep green color change (lala land=green=good= positive)

  • Negative: yellow (no color change)

7
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What does SIM media deep test for?

  • Presence of H2S production

  • Presence of tryptophanase which can hydrolyze tryptophan into Indole and pyruvic acid

  • Motility

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