Recombinant DNA and Biotechnology

Which of the following DNA sequences could be the recognition site for a restriction enzyme?

TGCGCA

Which of the following is not needed for DNA sequencing by the method we discussed in class?

Radioactive primer

What is the key enzyme used in PCR?

Taq DNA polymerase

The creation of a cDNA molecule relies upon

reverse transcriptase.

Which DNA sequence is a palindrome and thus might be a restriction enzyme site?

CCTAGG

To change a simple cloning vector (or plasmid) into an expression vector, we would need to add

a -10 and a -35 box.

Making a DNA copy of RNA

Reverse transcriptase

Cutting DNA backbone at a specific sequence

Restriction enzyme

Covalently joining a DNA fragment to a cloning vector

DNA ligase

Copying a specific region of DNA

Taq DNA polymerase

Not used in cloning

DNA helicase

Make covalent bonds between two DNA molecules

DNA Ligase

Not used in gene cloning

DNA polymerase

Cut expression vector to produce “sticky ends”

Restriction enzyme

Produce cDNA from mRNA

Reverse transcriptase

Not used in gene cloning

Helicase