TCA steps, enzymes

Pyruvate + Carbanion of TPP —> Hydroxyethyl-TPP

Pyruvate —> Acetyl CoA #1 enzyme: PDH E1

Hydroxyethyl-TPP +Lipoamide —> Acetyllipoamide + Carbanion of TPP

Pyruvate —> Acetyl CoA #2 enzyme: PDH E1

Coenzyme A + Acetyllipoamide —> Dihydrolipoamide + Acetyl CoA

Pyruvate —> Acetyl CoA #3 enzyme: Dihydrolipoyl Transacetylase E2

Dihydroamide + FAD —> Lipoamide + FADH2 —> FAD + NADH + H

Pyruvate —> Acety CoA #4 enzyme: Dehydrogenase E3

Produces NADH

Oxaloacetate + Acetyl CoA —> Citryl CoA + Citrate

TCA #1 enzyme: citrate synthase

Citrate —> cis-Aconitate —> Isocitrate

TCA #2 enzyme: Aconitase

Isocitrate —> Oxalosuccinate + NADH —> a-Ketoglutarate +CO2

TCA #3 enzyme: Isocitrate dehydrogenase

REGULATORY

Produces CO2 and NADH

A-ketoglutarate + NAD+ + CoA —> Succinyl CoA + CO2 + NADH

TCA #4 enzyme: a-ketoglutarate dehydrogenase complex

REGULATORY

Produces CO2 and NADH

Succinyl CoA + Pi + ADP —> Succinate + CoA + ATP

TCA #5 enzyme: Succinyl CoA

Thioester

Produces ATP

Succinate —> Fumarate + FADH2

TCA #6 enzyme : Succinate dehydrogenase

Produces FADH2

Fumarate —> L-Malate

TCA #7 enzyme: fumarase

Malware —> Oxaloacetate + NADH

TCA #8 enzyme: malate dehydrogenase

Produces NADH