lab procedures

• What is the medical term for red blood cell

Erythrocyte

• What is the proper term for capillary tubes

• -Hematocrit or microhematocrit

What is the liquid portion of blood that still contains clotting factors.

Plasma

What is the liquid portion of blood that is missing the clotting factors?

Serum

What parasite is known for causing cutaneous larval migrans?

• ancylostoma spp (hookworm).

What is the term for reddish colored plasma or serum ?

Hemolyzed

What is an FNA ( fine needle aspirate)?

• placing a needle into a mass or lymph node to extract cells for evaluation

Give an example of a chemical used to prevent blood from clotting.

Heparin

What is a reagent

Chemical used to run ELISA test kits

A serum/ plasma that is milky/ white is know to be?

Lipemic

A serum/plasma that is yellow is known to be?

• Icteric

What does putting a blood sample for a CBC in the refrigerator allow for?

Preservation of the sample so that it can be run later

A PCV is known as?

Hematocrit

What is the term for counting the percentage of leukocytes and type of leukocytes?

Differential

Where is a fecal sample stored while waiting for analysis?

Refrigerator

How do you determine the Total Protein of serum?

Albumin + globulin = TP

How is Dipylidium Caninum spread?

Ingestion of a flea

What is the infected host that is most likely to cause non-zoonotic tapeworm infection when ingested

Flea

What are the 2 main mites found in a skin scraping?

Demodex, Sarcoptic

What can be seen on a blood smear?

Reticulocytes, thrombocytes, RBC, WBC

Common intestinal parasites found in a fecal float?

Trichuris vulpis, Toxocara canis, coccidia,

What is the vector for heartworm?

Mosquitoes

What can cause lipemic plasma or serum?

Post-prandial sample, morbid obesity

What tests for kidney function and hydration?

BUN ( blood urea nitrogen)

What indicates azotemia in cats?

Elevated BUN or creat

What test gives the average glucose level over the life of a protein within the blood?

Fructosamine

What values are commonly used to evaluate the liver in small animals?

ALT(Alanine transaminase)/ ALKP(Alkaline phosphatase)

What values are used to evaluate the kidney function as a whole?

BUN, creat, sdma

What does amylase and lipase evaluate for?

Pancreatitis

How do you dispose of a needle and syringe used to draw blood?

Needle in sharps, syringe in biohazard container

What is hematuria?

Blood in the urine

What ectoparasites are barley visible to the eye?

Certain types of mites

What color tube tops produce serum after centrifuging?

red, sst, and white

What color tube top will produce plasma after centrifuging?

Purple, green

How do you apply a swab sample to a slide for evaluation

Roll the sample on the slide with a cotton tipped applicator

STT

Gel plug with clot activator that separated Prbc from serum

Blue TT

Contains NA Citrate

Purple TT

Contains EDTA Heparin

Green TT

Contains lithium Heparin

White TT

A Sterile tube with no additives

Red TT

Contains a clot activator with no gel plug

What can happen to a blood sample if it is spun for to long or to fast?

Sample becomes hemolyzed

Tube for what test

STT

Default tube for serum or reference lab

This type of tube is for what test

Blue TT

Blood coagulation profile Microhematocrit: PCV/TP

This tube for what type of test

Purple TT

CBC

This tube for what type of test

Green TT

Plasma for chemistry analysis

​White top: U/A

Which parasite life stage is looked for in a fecal float and can only be seen under a microscope?

OVA

Where do you find the Buffy coat

Between the RBC and the plasma

How do you collect a voided urine sample

With a ladle or bowl placed into the urine stream

Hat does the Buffy coat contain

WBC

How do you write a USG

1 decimal and 3 numbers after decimal

What part of the microhematocrit is used to evaluate the % of erythrocytes

Bottom of sample tray

Define Flocculant

Large amount of particles suspended in a urine sample

Where do you read a PCV

At the top of the red cells before the buffy coat

Where can blood be drawn from but is not usually used due to it being painful?

Toe nail

What color is the liquid used to read the Total protein?

Clear/light yellow or tan

What is the name of the centrifuge tube used to run a fecal or urinalysis and has a tapered end?

Conical tube

What is a possible zoonotic intestinal parasite?

Roundworm (toxocara)

What is each instrument used for

Refractometer

USG/TP

What is each instrument used for

Microscope

Magnifies things that can not be seen grossly

What is each instrument used for

Test strip

Presence of material via color change

What is each instrument used for

​Wrights Stain

Differentiate different cell types

How much sample should be drawn to run a lab test?

3 times the amount needed to run the test

What is each instrument used for

New Methylene Blue Stain

Identify reticulocytes

What is each instrument used for

Centrifuge

Used to separate liquids by weight

What is each instrument used for

Serology test

Test for the presence of an antigen or antibody in blood

What is each instrument used for

​Blood chemistry analyzer

Test for enzymes, hormones, other substance in serum

Ringworm can be identified about 50% of the time via what instrument?

Woods lamp

Define cystocentesis

Collecting a sterile urine sample from the bladder using a surgical puncture

What are the functions of the following cells

​Eosinophil

Responds to parasitic infection

What are the functions of the following cells?

​Lymphocyte

Assists body with antibody production, is most abundant in felines

What are the functions of the following cells?

​Basophil

Releases histamine during an allergic reaction

What are the functions of the following cells?

​Neutrophil

Acts as 1st responder and is most abundant in k9

What are the functions of the following cells?

Monocyte

Phagocytizes bacteria and debris

What are the functions of the following cells?

​Thrombocyte

Clotting components of blood

What chemistry value will change if you leave blood sitting to long before you spin it?

Glucose level will decrease

Define anuria

A lack of urine production

Who can handle a patient who might have rabies?

A person who is vaccinated for rabies

Describe each of the following

Differential blood smear

Analyze number shape size and type of cells

Describe each of the following

Basophil

​Rare cell to see on a blood smear

Describe each of the following

Morphology

Describes the cell structure, color shape size appearance

Describe each of the following

PCV/TP

part of a cbc but not included when reading a blood smear

Describe each of the following

Monolayer/Feathered edge-m

Area on blood film that is read for differential

What cell has a high nuclear-cytoplasmic ratio, coarse, clumping, dark-staining chromatin in its nucleus and sky bluecytoplasm?

Lymphocytes

What helps to establish diagnosis, track a disease coarse and offer a prognosis?

Laboratory results

What is used to clean up a liquid chemical spill?

Chemical absorbent pad

What fungus is commonly found in otitis externa

Malassezia

How do you clean up a spilled fecal float?

PPE, Absorbent to pick up liquid, detergent and disposable wipe

What is the order of diff quick stain use?

Fixative, eosinate, Polychrome blue

What is a normal k9 urine sample color?

Yellow

How long should a feacl float sit before reading it?

10 min

What is the proper way to collect and store a fecal sample?

Collect no more that 24hrs in advance, store in fridge

what is immersion oil in contact with?

Cells and oil immersion objective

What vessel is used to collect a large blood sample?

Jugular vein

What microscope objective is used to read a fecal or skin scrape?

Medium

What stain is used with a fecal smear?

Sedi-stain

What tubes are used for a CBC chemistry profile?

SST,LTT

What is the scientific name for a postmortem exam?

Necropsy

What color cap is for each?

Green

18G

What color cap is for each?

Pink

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