Blood Bank Lab Practical 1

RBC suspension, ABO typing, IAT.

1st step of RBC suspension?

Label tubes

2st step of RBC suspension?

Add 2 drop of EDTA whole blood

3rd step of RBC suspension? After filling the tube up with 3/4’s of saline…

Centrifuge for 1 minute

4th step of RBC suspension?

Pour ¾ of saline out and refill w/ saline

Make a RBC suspension:

Fill tube: How much and with what?

3/4’s of 0.9% Saline

ABO Typing:

To obtain plasma for ABO typing what must you do?

Remove plasma form patient sample

ABO Typing:

What is the name of the D control reagent?

Gamma clone ctrl

ABO Typing:

Which tubes get patient plasma?

Dctrl, A1 and B cell tubes

ABO Typing:

Which tubes get 1 drop of RBC suspension?

A, B, D, and Dctrl

ABO Typing:

What fluid should be added to the tubes first?

Add clear fluids first

ABO Typing:

How many drops of plasma in A1 & B cell tubes?

2 drops of plasma

ABO Typing:

How many drops of reagent in each tube?

1 drop

ABO Typing:

First step of ABO typing?

Label four tubes A, B, RhD, Dctrl, A1, B cellls.

ABO Typing:

Second step of ABO typing?

Add 1 drop of each anti- A, B, Drh and Dctrl reagent

ABO Typing:

Third step of ABO typing?

Add 1 drop of A1 and B cell reagent

ABO Typing:

Fourth step of ABO typing?

Add 2 drops of plasma to A1 and B cell tubes

ABO Typing:

Last step step of ABO typing?

Centrifuge for 30 seconds , read and record

IAT procedure:

What do you add to the auto-control before incubation?

Add 2 drops of patient plasma

1. Label 4 tubes _, _, _ and _________.

1, 2, 3, Auto-control

2. Add 1 drop of______ ________ cells to tubes 1, 2, and 3.

reagent screening

3. Add ____ drops of patient _____ to the tubes labeled I, II, III, and auto control.

2 drops; plasma

4. Add ____ drop of patient _____ to the Auto control tube.

1 drop; RBC

5. Centrifuge each incubated tube for _____ seconds and read for agglutination strength. This is the
   _______ ____ phase of reaction.

20 seconds; Immediate spin (RT)

6. Add ___ drop of a _____ to force a reaction. There is ____ or ____ as options.

2 drops; potentiators ; LISS ; PEG

7. _____ the tubes for ____ minutes. This is the beginning of the ____ reaction phase.

Incubate; 15 min ; 37C(body temp)

8. ____ each tube with ¾ of saline. Pour out saline(for blood button), refill and centrifuge ___ times for ___ minute.

Wash; 3 times; 1 minute

10. Add _____drops of ______(IgG) to each tube. Centrifuge for _______ seconds and read reaction.
    agglutination

2 drops; AHG; 20 sec;

11. To all ____________ AHG (IgG) reactions, add _____ drop of check cells. Centrifuge for ____ seconds.

negative; 1 drop; 20 sec

If AHG step comes out positive, what would you perform next?

ABO Screening (16 Reagents)