blood donation

below what pcv range for a dog and cat would indicate a need for a blood transfusion

dog: 15-20%, cat: 10-15%

what characteristics does a canine need to have in order to qualify to donate blood

healthy, >50#, 1-6 yrs, PCV >40%, current on vaccination

what blood tests are run before a canine donor can donate blood

CBC, chem panel, U/A, fecal, test for infectious agents (Ehrlichia, Babesia, RMSF, Borrelia, Brucella, Diroflaria)

how many DEA (dog erythrocytic antigens) types do dogs have

13 DEA’s

what blood type is the universal donor for dogs

type C and has only DEA 4

what are type A- in dogs missing

DEA 1.1,1.2,7

T/F dogs can be sedated for blood donation

false

what characteristics does a feline need to have order to qualify to donate blood

healthy, >10#’s, PCV >30-35%, current on vaccination, strictly indoors

what blood tests are run before a feline donor can donate blood

CBC, chem panel, U/A, fecal, test for Mycoplasma, FeLV, FIV(test twice 8-12 weeks apart), FIP

what is the most common blood type in felines

Type A

what does feline type B blood have in it

antiA antibodies

how much and how often can canines donate blood

10mls/#, q3 weeks

how many mls is one unit of canine blood

450 mls

what is the procedure for blood collection from a canine

surgical prep (shave, scrub), clamp tube, place needle in jug, unclamp tube, rock bag below patient, clamp tube before removing needle

what additive is in a 3 bag system that extends shelf life

adsol, extends by 20-37 days

how much and how often can felines donate blood

5 ml/#, q3-4 weeks

how many mls is one unit of feline blood

56 mls

what is the procedure for a feline blood donation

light anesthesia, no barbituates or acepromazine

surgical prep, draw in cat bags or 2-35 mls syringes with 19 ga butterfly catheter with 4 mls anticoagulant in each

what are the three types of collection techniques

plastic bag, glass bottle, syringe

what is the shelf life of blood with heparin

24h

what is 3.8% Sodium Citrate and what is its shelf life

just an anticoagulant, shelf life 24h

what is acid citrate dextrose (ACD) and what is its shelf life

anticoagulant and preservative, shelf life 21 days

what is citrate phosphate dextrose and what is its shelf life

anticoagulant and preservative, shelf life 21 days

what is citrate phosphate dextrose with adenine and what is its shelf life

anticoagulant and preservative, shelf life 35 days

what is the purpose of adding adenine to citrate phosphate dextrose

substrate for RBC’s to synthesize ATP during storage, improved viability of RBCs

how much plasma should be left in packed rbc’s

20% or 1 inch

indication for giving whole blood

hypovolemic anemic, hemorrhagic shock

what is the indication for giving packed RBC’s

oxygen carrying capacity, anemic normovolemic, IMHA, anemic hypovolemic in conjunction with fluids, anemic heart failure

what is the difference between FFP and stored plasma

FFP: frozen within 6-8 h of collection

stored: frozen after 6-8 h of collection

what is the indication for giving plasma

bleeding disorders and give passive immunity (neonates and sick/parvo puppy), hypoproteinemic, viral ds, warfarin tox

what should never happen to platelet rich plasma

never refridgerate, expires in 72 hours

what is the indication for platelet rich plasma

thrombocytopenia (temp fix)

what is the indication cryoprecipitate

hemophilia A, VWD, viral ds

what is used for a major cross match

2 drops donor red cell suspension, 2 drops recipient plasma

what is used for a minor crossmatch

2 drops donor plasma, 2 drops recipient red cell suspension

what is used for the control crossmatch

2 drops donor red cell suspension, 2 drops donor plasma

what are the two end point observations that would mean the donor and recipient are not compatible

hemolysis or agglutination

formula for recipient blood volume

dog: 88 ml/ body weight in kgs

cat: 66 ml/ body weight in kgs

technique of administering a blood transfusion

initially slow (0.11ml/#/min) for first 15 minutes to observe for reaction

what is a non-immunologic reaction and examples

not a reaction to red cell antigens

ex: sepsis, disease transmission, allergic reactions to foreign proteins (not erythrocytic antigens), circulatory overload, hypothermia

what is an immunologic reaction and examples

reaction to red cell antigens

ex: immediate hypersensitivity (salivation, v/d, dyspnea, death)

delayed hypersensitivity (days to weeks for expression, decreased PCV, hemoglobinuria)

what is oxyglobin

mass produced brand name of hemoglobin

adv and disadv of oxyglobin

adv: better at delivery of oxygen to tissues over red cells, works great on reversible anemias, shelf life up to 2 years stored at room temp

disadv: very expensive, doesn’t last long in the body (kidneys excrete)