E. coli Testing

MT65 sampling items

Bench trim

MT60 sampling items

Slaughter est beef manufactured trimmings intended for non intact use

What type of items fall under MT43 sampling

Ground beef, any location or source

MT64 ampling items

Other raw ground beef components

Cheek, head, weasand, heart, AMR, LTRFTB and ammoniated products

Est produces nonintact product, it’s transferred to another federal est for lethality, is it eligible for MT43 sampling? Should it be reflected in the HACCP?

No

Yes, verify the est’s hazard analysis and flow chart show product is intended for a controlled use AND est has controls to ensure the product is used as intended

Est doesn’t maintain clear records concerning end use of a product as intact or nonintact or if lethality is involved. Could IPP still collect a sample?

Yes, IPP assume products are intended for use in production of raw nonintact product

Product is subject to being sampled, est performs a lethality treatment, can IPP still sample?

Yes, IPP sample the product even if the est decides to change the intended use

How is sampling frequency determined? What components make up the frequency formula?

Max number of sampling tasks is based on est production volume (average daily volume in lbs per day)

What are some factors to consider when deciding what is a lot?

Scientific sampling programs for STEC the est uses to distinguish segments of production

Ssops/other prereq programs used to ctrl STEC spread/cross contamination btwn components during production

Processimg interventions limit/ctrl STEC contamination

Trim and components/rework carried from one production period to another

How much notice is required to est personnel before gathering samples?

Roughly 1-2 days’ notice, sufficient for the est to hold the sampled lot

Basic procedure for collecting an MT60/65 sample

Wash and dry to mid forearm

Open bag and invert to drop sterile cloth onto sample product

Place the open bag in an upright position on a clean, sanitary surface

Put on sleeves and gloves, (spray) sanitize both on arms

Unfold cloth, apply downward pressure and massage cloth along surface area of product (1.5 minutes per bin, 45-60 seconds per cloth side)

Refold cloth, place into original bag

Open and asceptically pour nBPW tube into the same bag

Gentle pressure to soak cloth in nBPW

Remove excess air and pack

Basic procedure for collecting an MT43 sample

Final packaging available, collect number of packages to equal 2lbs

Grab procedure for collecting an MT43 grab sample

Wash and dry hands

Open bag, place bag upright

Put on and sterilize gloves

Collect grab sample, enough to fill the 3 roll top bags to the fill line

Remove excess air from each bag - DO NOT tie the ends