CC-QA

Clinical Chemistry

-deals with the quantitative measurement

-numeric measurement of the certain analyte

• blood glucose level

• uric acid

• creatinine

-Test that is measured under clinical chemistry section

• blood

• urine

• cerebospinal fluid

• peritoneal fluid

• pleural fluid

• analysis of renal stone

-biological fluids

blood

-most common sample in clinical chemistry section

serum

-upon centrifugation of blood with non-additive tube we are able to yield

plasma

-upon centrifugation of blood with lithium heparin as an additive we are able to yield

Clinical Chemistry

-a major division of clinical laboratory

to perform analytic procedures that will yield accurate and precise results, aiding in patient diagnosis and treatment

-Primary purpose of Clinical Chemistry

Validation and Calibration

-calibration is done at the start of each new reagent lot

-periodically

Internal Quality Control

-daily done in the laboratory

-controls tested with every run

-plotted on Levey-Jennings chart

-2 level of controls (normal and pathological control)

-delta checks to help detect shifts and trends

Levey-Jennings Chart

-IQC is tested every run and is plotted on?

Normal and Pathological

-2 level controls

Delta checks

-helps detect shifts and trends in IQC

Proficiency testing

-example of External Quality Assessment

External Quality Assessment

-participation in proficiency testing compares results with other laboratories

Lung Center of the Philippines

-reference laboratory in the Philippines

Specimen Quality Assurance

-properly identify the patient and properly collect a sample, and properly handle the specimen

• Hemolysis

• Icterus

• Turbidity

-in SQA, lab. scientist should note the presence of any sample characteristics such as.

within 4 hours

-SQA, samples should be analyzed within

4 C for 8 hours

-SQA, if samples are analyzed later, it should be stored in?

-20 C

-SQA, for long term storage, samples should be frozen at

Potassium

-if sample is hemolyzed, this analyte is the most affected

Falsely Elevated

-what will result if there is a hemolyzed sample, the potassium will rise up, resulting in?

2-8 C

-For hematology, refrigerator temperature only

Serum or Plasma

-needs to be frozen

-specifically if long term storage

• Pipette

• Centrifuge speed

Equipment Maintenance/Calibration

3 to 4 times per year

-Pipette must be calibrated how many times

tachometer or strobe light

-Centrifuge speed must be checked using?

SYMBOLS

SYMBOLS

Lyophilized

-upon delivery of quality control materials of clinical chemistry they are in powdered form, also known as?

2-8 C

-temperature storage of lyophilized quality control materials

5 mL distilled water

-used to reconstitute lyophilized materials

10 days

-1 vial of quality control material lasts for how many days?