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RNA processing
RNA processing
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26 Terms
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1
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the steps required to produce a useable mRNA
what is RNA processing
2
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prokaryotes, the transcripts are ready for translation
which type of cell (prokaryotes or eukaryotes) have no RNA processing necessary
3
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yes, must be processed and transported to cytoplsasm
Do eukaryotes need to be transcribes
4
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1. additon of a 5' cap
2. additon of a poly- A tail at 3'
3. splicing to remove introns
name the 3 steps of RNA processing
5
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RNA processing is at the RNA stage of transcription
RNA processing is at what stage or transcription- translation (DNA, RNA, or Protein)
6
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there is a methylated cap added to the 5' end and growing pre-mRNA strand during transcription
capping
7
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1) promotion of translation \n 2) regulation of export \n 3) prevention of exon nucleus degradation
name the functions of capping
8
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enzymes outside of nucleus that break down nucleic acids (RNA)
what does exonucleus degredation mean
9
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polydenylation
what does Poly-A stand for
10
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occurs after pre-mRNA is cleaved from DNA template strand a secondary enzyme PAP adds a long chain of adenine base
polydenylation
11
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polydenylate polymerase
what does PAP stand for
12
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degradation of RNA transctipt
what does poly-A tail prevent
13
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introns and extrons
eukaryotic genes are a mixture of
14
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expressed segment of the gene, these sequences are transcribed and translated into a protein
extron
15
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an intervening segment of the gene, these sequences are transcribed but NOT translated
intron
16
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spliceosomes
RNA splicing is carried out by \___________
17
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proteins and small RNA fragments
RNA splicing is a mixutre of
18
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responcible for removing intron segments of the RNA transcript and joining the remaining exons together
describe RNA splicing and its functions
19
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a single gene can code for more thatn one different protein, the inclusion or exclusion of exons can change expression
alternative splicing
20
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1) there's a 5' cap \n 2) 5' un-translated region \n 3) protein coding sequence \n 4) 3' un-translated region \n 5) Poly-A tail
what's left post processing
21
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un-translated region
what does UTR stand for
22
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during transcription
when is the methylated cap added onto the RNA strand
23
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the methylated cap allows it to leave
how can the RNA leave the nucleus
24
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the 3' end
where is the poly-A tail added on
25
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a single gene can code for mote than one protein. the amount of exons included can change the expression of the gene (called alternative splicing)
why are introns important ot have even though they are taken out
26
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recognizes intron segments, cuts them, reconnects exons together
what is the spliceosomes role in RNA splicing
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