BIO-123: Eukaryotes Practical

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Saccharomyces cerevisiae plate

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Saccharomyces cerevisiae type of eukaryote

fungus

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Saccharomyces cerevisiae y/m

yeast - has buds instead of hyphae, smooth

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Saccharomyces cerevisiae classification basis

sexual reproduction

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Saccharomyces cerevisiae # of cells

unicellular

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diseases caused by Saccharomyces cerevisiae

“yeast infections” or candidiasis caused by Candida albicans

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Saccharomyces cerevisiae transmission

Endogenous; member of the normal microbiome but can overgrow if one is immunocompromised or taking antibiotics that kill good bacteria but leave the yeast behind to flourish

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Saccharomyces cerevisiae signs/symptoms

irritation, itching; inflammation at infection site

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Saccharomyces cerevisiae diagnosis

finding budding yeasts in samples

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Saccharomyces cerevisiae treatment

azoles

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Rhizopus stolonifer plate

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Rhizopus stolonifer type of eukaryote

fungus

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Rhizopus stolonifer y/m

mold - has hyphae instead of buds

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Rhizopus stolonifer classification basis

sexual reproduction

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Rhizopus stolonifer # of cells

multicellular

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Rhizopus stolonifer disease

mycosis - a disease caused by fungi

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Rhizopus stolonifer trasmission

inhalation of spores

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Rhizopus stolonifer signs/symptoms

systemic mycoses - coughing and/or skin rashes

cutaneous/subcutaneous mycoses - skin rashes and irritation

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Rhizopus stolonifer diagnosis

Finding budding yeasts/pseudohyphae or encapsulated spores in skin scrapings or tissue biopsies

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Rhizopus stolonifer treatment

azoles

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toxoplasma gondii slide

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toxoplasma gondii lifecycle stages

cyst and trophozoite

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toxoplasma gondii classification basis

motility (movement)

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toxoplasma gondii # of cells

unicellular

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toxoplasma gondii disease

Toxoplasmosis– which is transmitted by cats and is dangerous to humans if it crosses the placenta

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toxoplasma gondii transmission

ingesting or inhaling cysts from cat poop; cross-placental transmission to fetuses

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toxoplasma gondii signs/symptoms

some have no symptoms; some may have flu-like symptoms, but can cause miscarriage in pregnant woman

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toxoplasma gondii diagnosis

finding cysts in tissue samples, trophozoites in blood smears, antibody tests

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toxoplasma gondii treatment

Anti-protozoals (pyrimethamine)

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plasmodium smear (ring form)

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plasmodium type of eukaryote

protozoa

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plasmodium lifecyle stages

cyst and trophozoite

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plasmodium classification basis

motility

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plasmodium # of cells

unicellular

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plasmodium disease

malaria

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plasmodium transmission

mosquitoes

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plasmodium signs/symptoms

Waves of fever and chills that coincide with the parasite’s lifecycle

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plasmodium diagnosis

finding trophozoites trapped in RBCs

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plasmodium treatment

anti-protozoals like chloroquine

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Saccharomyces cerevisiae slide

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Amoeba proteus slide

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Amoeba proteus type of eukaryote

protozoa

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Amoeba proteus lifecycle stages

cysts and trophozoites

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Amoeba proteus classification basis

motility

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Amoeba proteus # of cells

unicellular

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Amoeba proteus disease

Entamoeba histolytica – causes amoebiasis / amoebic dysentery

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Amoeba proteus transmission

Drinking contaminated water containing cysts

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Amoeba proteus signs/symptoms

bloody diarrhea

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Amoeba proteus diagnosis

finding cysts in stool samples

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Amoeba proteus treatment

Anti-protozoals like nitroimidazole

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Giardia lamblia slide

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Giardia lamblia type of eukaryote

protozoa

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Giardia lamblia lifecycle stages

cyst and trophozoite

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Giardia lamblia classification basis

motility

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Giardia lamblia # of cells

unicellular

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Giardia lamblia disease

Giardiasis

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Giardia lamblia transmission

Drinking water contaminated filled with cysts. Cysts survive stomach acid and make it to intestine where trophozoites cause inflammation.

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Giardia lamblia signs/symptoms

Greasy/shiny/floaty diarrhea

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Giardia lamblia diagnosis

Finding cysts and trophozoites in stool sample

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Giardia lamblia treatment

Anti-protozoals like nitroimidazole

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Schistosoma egg slide

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Schistosoma type of eukaryote

helminth (worm)

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Schistosoma lifecycle stages

egg, larva, adult

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Schistosoma classification basis

body shape

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Schistosoma # of cells

multicellular

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Schistosoma disease

Schistosomiasis

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Schistosoma transmission

swimming in contaminated water where the larval form burrows into your skin

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Schistosoma signs/symptoms

Itchy spots on the skin but also can cause damage to other organ systems.

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Schistosoma diagnosis

Finding eggs in stool, urine, or tissue samples. Elevated eosinophil count.

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Schistosoma treatment

Anti-helminthics like praziquantel

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Schistosoma egg

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Enterobius vermicularis egg slide

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Enterobius vermicularis type of eukaryote

helminth (worm)

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Enterobius vermicularis lifecycle stages

egg, larva, adult

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Enterobius vermicularis classification basis

body shape

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Enterobius vermicularis # of cells

multicellular

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Enterobius vermicularis disease

pinworm

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Enterobius vermicularis transmission

Contacting eggs from scratching itchy anal folds or from contaminated friends/family and ingesting them. Fecal-oral route.

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Enterobius vermicularis signs/symptoms

itchy anus

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Enterobius vermicularis diagnosis

finding eggs in anal folds

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Enterobius vermicularis treatment

Anti-helminthics like albendazole

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Taenia worm specimen

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Taenia type of eukaryote

helminth (worm)

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Taenia lifecycle stages

egg, larva, adult

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Taenia classification basis

body shape

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Taenia # of cells

multicellular

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Taenia disease

tapeworm disease

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Taenia transmission

Ingesting larval cysts called cysticerci in contaminated meat

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Taenia signs/symptoms

Decreased appetite, weight loss, diarrhea, malnutrition

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Taenia diagnosis

Finding eggs in the stool or elevated eosinophil count

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Taenia treatment

Anti-helminthics like praziquantel

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Ascaris egg

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Ascaris type of eukaryote

helminth (worm)

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Ascaris lifecycle stages

egg, larva, adult

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Ascaris # of cells

multicellular

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Ascaris disease

Ascariasis or intestinal roundworms

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Ascaris transmission

Ingesting eggs from contaminated food or water

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Ascaris signs/symptoms

Diarrhea, weight loss, intestinal blockages; can also migrate throughout the body and affect lungs

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Ascaris diagnosis

Finding eggs in stool, high eosinophil count

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Ascaris treatment

Anti-helminthics like mebendazole

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