6. Lentiviral CRISPRCas9 vector mediated miR-21 gene editing inhibits the epithelial to mesenchymal transition in ovarian cancer cells

Lentiviral CRISPR/Cas9 Vector Mediated miR-21 Gene Editing Inhibits the Epithelial to Mesenchymal Transition in Ovarian Cancer Cells

What is the title of this study?

The epithelial to mesenchymal transition

What process is being inhibited in ovarian cancer cells in this study?

EMT

embryogenesis, wound repair, and cancer

Epithelial-mesenchymal transition

What does EMT stand for?

identify potential of miR-21 as biomarker for cancer

objective of this study?

ovarian cancer

poor patient survival and high chances of relapse and drug resistance because of late diagnosis

use CRISPR/Cas9 to mutate miR-21 and disrupt its regulatory functions in tumor expression

main methodology in the study

1. cell culture (DMEM and FBS)

2. lentiviral vector production

3. transduction and selection (Puromycin)

4. genomic analysis

5. expression analysis

6. functional assays

7. EMT marker analysis

8. Statistical analysis

methodology steps

• western blotting

• immunofluorescent staining

two kinds of EMT marker analysis

Student’s t-test with p<0.05 considered significant

statistical analysis used?

• highly efficient introduction of mutations in precursors miR-21 sequences

• inhibition of cell proliferation, migration and invasion in ovarian cancer cells

• sensitized chemotherapy drug treatment

• inhibition of EMT in ovarian cancer cells

results of the study

• Utilization of Lentiviral CRISPR/Cas9

• further investigation of off-target effects

• assessment of miRNA functions

• potential for clinical applications

What are some recommendations for applications based on this study?