4 - Gene and Genome Structure, Transcription and Translation

Complementation

Determines if mutations are on same or different genes

• Complementation only occurs with different genes

If the mutants DON’T produce wildtype…

They DO NOT COMPLEMENT one another, the mutations are in the SAME GENE

If the mutants DO produce wildtype…

They DO COMPLEMENT one another, the mutations are in DIFFERENT GENES

Complementation Chart

(+)= wild-type (mutations on different genes- complementation)

(-)= mutant (mutation on same gene- no complementation)

What are the building blocks for RNA?

NTP

What are the building blocks for DNA?

dNTP

Purines

Have a double-ring structure; A and G

Pyrimidines

Have a single-ring structure; C, U, and T

What type of reaction is Phosphodiester linkage?

Condensation

DNA’s Double Helix Structure

Bases in interior regions; Hydrophilic sugar-phosphate backbone

on exterior; Anti-parallel strands of DNA, Bases are paired purine-pyrimidine,

A-T, G-C