DNA CONCENTRATION AND PURITY
0.0(0)
Studied by 3 people0.0(0)
Call Kai
Learn
Practice Test
Spaced Repetition
Match
Flashcards
Knowt PlayCard Sorting
1/7
There's no tags or description
Looks like no tags are added yet.
Study Analytics
Name | Mastery | Learn | Test | Matching | Spaced |
|---|
No study sessions yet.
8 Terms
1
New cards
A260/A280: 1.8 - 2.0 (pure DNA)
A260/A230: 2.0 - 2.2 (low salt/solvent contamination)
What is the positive reaction of this test?
2
New cards
A260/A280: < 1.8 (protein contamination) or > 2.1 (RNA contamination)
A260/A230: < 1.8 (salt, phenol, EDTA, carbohydrate contamination)
What is the negative reaction of this test?
3
New cards
Tris-EDTA buffer
What is the reagent of this test?
4
New cards
Estimate DNA concentration and assess DNA purity
Principle
5
New cards
Tris-EDTA buffer
6
New cards
Dissolves and becomes stabilized
What is the reaction if the DNA isolate when reacted with TE buffer?
7
New cards
Maintains a stable pH
Function of the Tris component
8
New cards
EDTA chelates magnesium ions that are necessary for DNase enzymes to degrade DNA
Function of EDTA
Note 
Flashcards (20)