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Independent variable
The variable that is changed on purpose; placed on the x-axis.
Dependent variable
The variable that is measured; placed on the y-axis.
Graph title format
Written as "Dependent variable vs. Independent variable."
Graph requirements
Includes title, labeled axes, units, proper scaling, plotted data, and trend line when needed.
When to use a line graph
Used to show changes over time or continuous data.
When to use a bar graph
Used to compare categories or groups.
Trend line
Showcases the general pattern or direction in data.
Identifying data points
Finding specific values directly from a graph or table.
Describing trends
Explaining patterns such as increasing, decreasing, or constant changes.
Describing relationships
Explaining how one variable affects another.
Mean
The average of data values; sum of values divided by number of values.
Percentage change
(new value − old value) ÷ old value × 100.
Rate of change
Change in a variable over time (Δy/Δt).
Ratio
Comparison of two numbers or quantities.
Water potential equation
Ψ = ΨP + ΨS (water potential = pressure potential + solute potential).
Solute potential equation
ΨS = −iCRT.
Ionization constant (i)
Number of particles a solute forms in water (e.g., NaCl = 2).
Gas constant (R)
R = 0.0831 L·bar/mol·K.
Temperature in Kelvin
Calculated using T(K) = °C + 273.
Gibbs free energy equation
ΔG = ΔH − TΔS.
Negative ΔG
Indicates a spontaneous, exergonic reaction.
Positive ΔG
Indicates a non-spontaneous, endergonic reaction.
Chi-square formula
χ² = Σ((O − E)² / E).
Observed value (O)
The actual measured data from an experiment.
Expected value (E)
The predicted or theoretical value.
Degrees of freedom
The number of categories minus one (n − 1).
Critical value
Used to determine if results are statistically significant.
Rejecting the null hypothesis
Occurs when χ² > critical value; results NOT due to chance.
Failing to reject the null hypothesis
Occurs when χ² < critical value; results are due to chance.
Null hypothesis (H0)
States that there is no difference, no effect, or differences are due to random chance.
Alternative hypothesis
States that there IS a difference or an effect.
Standard error
The standard deviation of the sample mean; measures accuracy.
Small standard error
Indicates consistent data and reliable mean.
Large standard error
Indicates variable data and less reliable mean.
Error bars
Graphical display of standard error on data points.
Overlapping error bars
Indicate no significant difference between groups.
Non-overlapping error bars
Indicate a statistically significant difference.
Scientific claim
A testable statement based on observations or data.
Evidence
Data or observations that support a claim.
Reasoning
Explanation connecting evidence to biological principles.
Connecting results to biology
Relating experimental outcomes to concepts like enzymes, diffusion, respiration, etc.