BIOL LAB Practical #3

LAB Assignments + Pre-Lab Quizzes

What are genes?

Genes are DNA molecules that contain the code to ultimately make proteins.

Match the nitrogenous base to the complementary base for DNA. 

A —> T

C —> G

G —> C

T —> A

The genetic code is said to be redundant. What does this mean?

Some codons code for the same kind of amino acid

The genetic code is universal, meaning all living things (i.e. bacteria, protist, fungi, plants, and animals) use the same basic genetic code. 

If we are all using the same genetic code, then how are living things so different?

 There are numerous combinations in the order of the genetic sequence

What is responsible for adding the complementary bases from a DNA template strand to build the mRNA?

RNA polymerase

What is the purpose of a codon?

A codon is a set of three nitrogenous bases that are read as one unit during translation that code for a specific amino acid.

_____ is responsible for "reading" the codons contained within the mRNA. 

tRNA

If the DNA template sequence is
3' TACGATCTGACAATT 5'

What will be the mRNA produced?

AUGCUAGACUGUUAA

If the mRNA is:
AUGCUAGACUGUUAA

What would be the tRNA anticodon sequence?

UACGAUCUGACAAUU

From the mRNA sequence, you can determine the amino acid sequence that will be produced.

Using the following mRNA sequence: 

AUGCUAGACUGUUAA

What will be the resulting Amino Acid sequence?

1. Met

2. Leu

3. Asp

4. Cys

5. STOP  

Gel Electrophoresis is used to separate which of the following?

 DNA, RNA, & Proteins

Which property determines the direction molecules will move through a gel?

Charge

True or False: 

DNA is positively charge, so it will travel to the negative end of the gel.

False

Molecules with a ___ mass will travel further through a gel.

Smaller

True or False: 

DNA fingerprints would be very similar between two distantly related people. 

False