Topic 4: Transfer of Bacteria using Aseptic Technique

Cultures

________ can be transferred with sterile cotton swabs, pipettes, glass rods or syringes.

Inoculating loop

end of nichrome wire is bent into a loop

Inoculating needle

end of nichrome is straight

What is used to exlude contaminants?

aseptic techniques

True or False: It is okay to leave test tubes and petri dishes open when not working on them.

False - only open when working

What is the benefit of broth cultures?

provide a large number of bacteria in a small space and easily transported

What are benefical of agar slants?

provide a solid growth surface, easier to store and transport than petri dishes

Agar deeps are used to culture bacteria that require less ___.

oxygen

Semisolid agar deeps contain ___ agar instead of usual ___ agar and is used to determine whether a bacterium is motile.

0.5-0.7%, 1.5%

What is a inoculating loop?

end of nichrome wire is bent into a loop

What is inoculating needle?

end of nichrome wire is straight

True or False: Cultures can be transferred with sterile cotton swabs, pipettes, glass rods, or syringes.

True