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Ch.1
List the 6 common bacterial shapes
Coccus
Bacillus
Vibrio
Cocco-bacillus
Spirillum
Spirochete
Ch. 1
Which ones are spherical? Curved? Rod-Shaped?
Spherical: Coccus
Curved: Spirillum, Spirochete, Vibrio
Rod-shaped: Bacillus
Ch. 1
What is the major defining factor characteristic of eukaryotes?
Contains a nucleus
Ch. 1
Name 2 types of protists?
Algae + Protozoa
Ch. 1
Which one has cells surrounded by cell walls of cellulose?
Plants
Ch. 1
Agar, a gel used to grow microorganisms in a Petri dish is derived from ______?
Algae
Ch. 2
Define the following:
- Wavelength
- Amplitude
- Frequency
Wavelength: distance between 2 points
Amplitude: height or depth of each peak
Frequency: rate of vibration OR # of wavelengths
Ch. 2
Describe the following terms:
- Magnification
- Resolution
- Contrast
Magnification: enlarging the image
Resolution: to be able to tell that 2 separate points are indeed separated
Contrast: detecting different structures in a specimen
Ch. 2
Define numerical aperture
The measure of a len's ability to gather light
the higher the numerical aperture, the better the resolution
Ch. 2
Name 2 factors that affect resolution
1) shortening the wavelength
2) increasing the numerical aperture
Ch. 2
Describe the relationship between numerical aperture and resolution
The higher the numerical aperture, the better the resolution
Ch. 2
Describe the relationship between wavelength and resolution
Short wavelengths= increased resolution power
Ch. 2
The 2 types of electron microscopes are _______ and _________
SEM (scanning electron microscope) and TEM (transmission electron microscope)
Ch. 2
An electron microscope has a higher resolution, why?
It uses short-wavelength electron beams, instead of light
Ch. 2
Which of the following techniques of EM is used to observe the 3D surface details of the specimen?
TEM
Ch. 2
Which of the techniques creates an image defecting by electrons that are passing through the specimen?
TEM
Ch. 2
Which of the techniques of EM offers valuable inform action about detailed internal structures of the specimen?
SEM
Ch. 2
Which of the techniques creates an image by detecting electrons reflected from the surface of the sample?
SEM
Ch. 3
What does the modern endosymbiotic theory state?
That mitochondria and chloroplasts arose as a result of prokaryotic cells establishing a symbiotic relationship within a eukaryotic host
Ch. 3
What is plasmolysis?
contraction of plant cells, which lead to shrinking
Ch. 3
A glycocalyx is a sugar coat, of which there are 2 important types:
_______ and _________
Capules and Slime layers
Ch. 3
Which of the 2 types of glycocalyx types contribute to a microbe's pathogenicity?
Capsule and slime layer
Ch. 3
What is Osmosis?
The diffusion of water
*Low concentration= more water
*High concentration= less water
Ch. 3
Differentiate between an isotonic, hypertonic, and hypotonic medium
Isotonic: same solute as another solution (no change in shape)
Hypertonic: higher solute concentration (shriveled up in shape)
Hypotonic: lower solute concentration (inflated shape)
Ch. 3
What is the direction of water flow for a bacterial cell living in a hypotonic, hypertonic, and isotonic environment?
Isotonic: no net movement
Hypertonic: out of the cell
Hypotonic: into the cell
Ch. 3
What advantages do cell walls provide prokaryotic cells in a hypertonic environment?
- allows to maintain its shape longer
- prevents the cell membrane from expanding
Ch. 3
What evidence supports the endosymbiotic theory?
- mitochondria and chloroplasts are derived from the uptake of bacteria
- these bacterias establish a symbiotic relationship w/ their host cells so that it can eventually lead to the bacteria evolving into mitochondria and chloroplasts
Ch. 3
Briefly describe the functions of a nucleoid
Nucleoid:
where prokaryotic DNA interacts w/ nucleoid-associated proteins (NAPs) that assist w/ the organization and packaging of the chromosomes
Ch. 3
Describe Plasmids
Plasmids:
Carries genes that confer advantageous traits such as antibiotic resistence
Ch. 3
Describe Ribosomes
Ribosomes:
Responsible for protein synthesis
Ch. 3
Describe Inclusions
Inclusions:
they are various nutrients or pigments that DO NOT have any activity
Ex: glycogen, lipids, melanin (pigment)
Ch. 3
Describe Endospores
Endospores:
allows bacterium to produce a dormant and highly-resistance cell to preserve the cell's genetic material during times of stress
Ch. 3
Describe Cell Walls
Cell wall:
protects the cell from harsh conditions in the outside environment
Ch. 3
Describe Flagella
Flagella:
structures used by cells to move in aqueous environments- acts like propellers to move
Ch. 3
Describe F Pili
F Pili:
when 2 cells physically transfer or exchange parts of their respective genome
Ch. 3
Describe Fimbriae
Fimbriae:
short-bristle proteins enables a cell to attach to surfaces and to other cells
Ch. 3
Compare flagella's rotation during running and tumbling of bacteria?
Running: flagella rotates in a COUNTER-CLOCKWISE direction (bundled flagella)
Tumbling: split/separated in a CLOCKWISE direction
Ch. 3
Compare and contrast monotrichous, amphitrichous, lophotrichous, and peritrichous flagella?
Monotrichous: bacterium w/ singular flagellum, located at the end of one cell
Amphitrichous: flagellum or tufts at both ends of the cell
Lophotrichous: has a tuft at one end of the cell
Peritrichous: flagella that covers the entire surface of a bacterial cell
Ch. 4
List the 5 types of symbiotic relationships
1) Mutualism
2) Amensalism
3)Commensalism
4) Neutralism
5) Parasitism
Ch. 4
Describe Mutualism population?
when 2 species benefit from each other
pop. A= benefitted
pop. B= benefitted
Ch. 4
Describe Amensalism population?
when 1 population harms another but remains unaffected
pop. A= harmed
pop. B= unaffected
Ch. 4
Describe Commensalism population?
when 1 organism benefits and the other is unaffected
pop. A= benefitted
pop. B= unaffected
Ch. 4
Describe Neutralism population?
when neither organism is affected in an y way
pop. A= unaffected
pop. B= unaffected
Ch. 4
Describe Parasitism population?
when 1 organism benefits while harming another
pop. A= benefitted
pop. B= harmed
Ch. 5
What do monoecious and dioecious mean?
Monoecious: having both female and male reproductive organs in the same individuals
Dioecious: having both female and male reproductive organs in separate individuals
Ch. 5
Are nematodes segmented?
No, they are unsegmented
Ch. 5
Differentiate between the 3 growth forms of lichens:
- Crustose lichens
- Foliose lichens
- Fruticose lichens
Crustose: tightly attached, crusty appearance
Foliose: leaf-like lobes
Fruticose: rounded structures of overall branched appearance
Ch. 5
What types of organisms are found in lichens?
Green Algae (cyan bacterium) and Fungus
Ch. 5
Whereas animals have cholesterol in their cell membranes, fungal cell membranes have different sterols called ________,
which are often exploited as targets for anti fungal drugs
Ergosterols
Ch. 5
Fungal cell walls contain __________, as opposed to cellulose found in the cell walls of plants and many protists.
Chitin
Ch. 5
Most multicellular fungal bodies are made up of filaments called ________, that can form a targeted network called a ________
- Molds
- Hyphae
Ch. 5
What does dimorphic mean?
Having more than one appearance during their cell cycle
Ch. 5
Differentiate between:
(a) hyphae and mycelium
Hyphae:
multicellular fungal body made up of filaments
Mycelium:
tangled network of fine, white filaments
Ch. 5
Differentiate between:
(b) septate and non-septate hyphae
Septate:
consists of walls between cells
Non-septate:
LACKS walls and cell membranes between the cells
Ch. 5
Among platyhelminths, the flukes and tapeworms are medically parasites.
Which of the two are segmented?
Flukes (cestodes) and Tapeworms (trematodes)
Ch. 5
Phylum Platyhelminths are flatworms that includes the ____________, _________, and the _____________, which includes planarians.
1) Flukes
2) Tapeworms
3) Turbellarians
Ch. 5
What is the most common nematode infection in the U.S.?
Pinworm
Ch. 5
The 2 major groups of parasitic helminths are ___________ and ____________.
1) Nematoda
2) Platyhelminths
Ch. 6
Describe the general characteristics of viruses as pathogens?
- lacks genes
- infectious, acellular pathogens
- DNA or RNA genome (never both)
- genome is surrounded by protein
- travel lunar parasites w/ host and cell-type specificity
Ch. 6
Which types of viruses have spikes?
1) Hemaglutinin (H)
2) Neuraminidase (N)
Ch. 6
Each capsid is composed of protein subunits called?
capsomeres
Ch. 6
Differentiate between naked viruses and enveloped viruses?
Naked Viruses:
form only a nucleus acid and capsid
Enveloped Viruses:
small portion of phospholipid membrane "budding" w/ host cell
(can be intracellular or cytoplasmic in origin)
Ch. 6
List and describe the 5 stages in. the bacterio lyctic cycle and briefly describe what happens at each stage?
1) Attachment:
attached to the surface of the host cell
2) Penetration:
viral DNA enters host cell
3) Biosysnthesis:
phage DNA replicates and phage proteins are made
4) Maturation:
new phage of particles are assembled
5) Lysis:
releases newly made phages
Ch. 6
(a) Do bacteriophages have a lyctic or lysogenic cycle?
(b) Which one leads to the destruction of the host cells?
(a) Either or
(b) Lysis
Ch. 6
Which one leads to integration of phage into the host genome?
lysogenic
Ch. 6
What is transduction?
when a bacteriophage transfers DNA from one bacterium to another during sequential Infections
Ch. 6
Discuss the difference between generalized and specialized transduction?
Generalized:
when a random piece of bacterial chromosomal DNA is transferred by the phage during the lytic cycle
Specialized:
occurs at the end of lysogenic cycle, when the prophage is exercised and bacterium enters the lytic cell
Ch. 6
Differentiate between:
- persistent infections
- latent infections
- chronic infections
Persistent infections:
not completely cleared but remains in certain tissues for organs of the infected person
Latent infections:
capable of remaining dormant (latency)
Chronic infections:
recurrent or persistent over a long time (or lifelong)
Ch. 7
Differentiate between macronutrients and micronutrients (or trace elements) ?
Macro:
large amounts; proteins, fats carbohydrates, and water
Micro:
small amounts; vitamins and minerals
Ch. 7
Why is the concept of isomerism very important in chemistry?
bc the structure of a molecule is always directly related to its function
Ch. 7
Differentiate between structural isomers and stereoisomers?
Structural:
compounds w/ identical molecular formulas but differ in bonding sequence of atoms
Stereoisomers:
Isomers that differ in the spatial arrangements of atoms
Ch. 7
What are enantiomers?
- they exhibit chirality
these chemical structures are non superimposable mirror images of each other
Ch. 7
Which one(s) is/are linear and which one(s) is/are branched for the following:
- Starch
- Glycogen
- Cellulose
Linear: Cellulose
Branched: Glycogen and Starch
Ch. 7
Which one(s) is/are energy storage molecule in animals and bacteria?
In plants?
Animals: Glycogen
Plants: Starch
Bacteria: Glycogen
Ch. 7
Which one(s) is/are formed from just 2 types of compounds: glycerol and fatty acids?
- Triglycerides
- Phospholipids
- Isoprenoids
- Sterols
Triglycerides
Ch. 7
Differentiate between primary, secondary, tertiary and quaternary structures of proteins?
Primary:
sequence of a chain of amino acids
Secondary:
local folding of polypeptide chain
Tertiary:
3D folding pattern
Quaternary:
consists of more than one amino acid chain
Ch. 7
Differentiate between the native and the denatured protein?
Native:
folded proteins that are fully functional in their normal role
Denatured:
When a protein loses its shape (3D) and is no longer functional
Ch. 7
What makes carbon so suitable to be part of all the macromolecules of living organisms?
- has unique properties
- can form covalent bonds to as many as 4 different atoms
- "backbone"
Ch. 7
Differentiate between these 4 types of disaccharides:
- sucrose
- lactose
- galactose
- maltose
Sucrose:
Naturally occurring in fruits and vegetables
(Glucose + Fructose)
Lactose:
Dairy products
(Glucose + Galactose)
Galactose:
found in milk, and always linked to glucose
(Galactose + Glucose)
Maltose:
doesn't occur in naturally occurring foods- except for sprout grains
(Glucose + Glucose)
Ch. 7
________ the primary components of adipose tissue (body fat) are major constituents of sebum (skin oils)?
Triglycerides
Ch. 7
Which one(s) is/are the important components of plasma-membrane?
(a) triglycerides
(b) phopholipids
(c) isoprenoids
(d) sterols
(b) phospholipids
(such as: lipids, proteins, carbohydrates)
Ch. 7
________ forms a lipid-bilayer sheets that makeup the cell membranes of nearly all organisms.
plasma membrane
Ch. 7
What are the differences between organic and inorganic molecules?
Organic:
- larger and more complex
- held together by covalent bonds to form cells of an organism to perform life functions
Inorganic:
Opposite of what organic molecules do
Ch. 7
What are isomers?
molecules w/ the same atomic makeup but different structural arrangement of atoms
Ch. 7
List the 6 most abundant elements in a cell that account for about 99% of the dry weight of cells?
1) Hydrogen (H)
2) Carbon (C)
3) Oxygen (O)
4) Nitrogen (N)
5) Phosphorus (P)
6) Sulfer (S)
Ch. 8
Differentiate between:
(a) autotrophs and heterotrophs
Autotrophs:
convert inorganic CO2 into organic compounds
Heterotrophs:
rely on complex organic compounds as nutrients
Ch. 8
Differentiate between:
(b) phototrophs and chemotrophs
Chemotrophs:
obtain their energy for electron transfer by breaking chemical bonds
Phototrophs:
get their energy for electron transfer from lipids
Ch. 8
Differentiate between:
(c) organotrophs and lithotrophs
Organotrophs:
- are chemotrophs that obtain energy from organic compounds
(ex: humans, fungi, etc..)
Lithotrophs:
get their energy from inorganic compounds
Ch. 8
Differentiate between;
(d) oxidation reactions and reduction reactions
Oxidation reactions:
removes electrons from donor molecules
Reduction reactions:
add electrons to acceptor molecules
Ch. 8
(a) What it glycolysis?
(b) Where in the cell does it happen?
(a) Glycolysis:
most common pathway for the catabolism of glucose (producing energy)
(b) it happens in the..
- energy investment phase &
- energy payoff phase
Ch. 8
Overall, in this process of glycolysis, the net gain from the breakdown of a single glucose molecule is 2________, 2___________, and 2__________.
2 G3P
2 BPG
2 Pyruvate
Ch. 8
Where in the cell does the Kreb cycle take place in prokaryotes and in eukaryotes?
Prokaryotes: Cytosol
Eukaryotes: matrices of the mitochondria
Ch. 8
Differentiate between homolactic fermentation and heterolactic fermentation?
Homolactic:
1 molecule of glucose= 2 molecules of lactic acid
Heterolactic:
Yields, CO2 and ethanol in addition to lactic acid
Ch. 8
Briefly describe how..
- competitive inhibitors
- noncompetitive inhibitors
- allosteric activators
- feedback inhibition
regulate enzyme activity?
Competitive:
binds to enzyme's active site to prevent binding to its substrate
Non-competitive:
binds to enzyme at a location other than its active site
Allosteric:
also binds to location other than active site, but produces positive feedback
Feedback inhibitors:
regulates its OWN product
Ch. 9
List the 4 phases of bacterial growth curve?
1) "lag"
2) "log"
3) "stationary"
4) "death"
Ch. 9
Differentiate between the 4 phases of bacterial growth curve?
1) "lag"
- stays flat
2) "log"
- increases
3) "stationary"
- no action
4) "death"
- "falls" to its death
Ch. 9
Describe different categories of microbes w/ temperature requirements for growth
(a) psychrophile
(b) meophile
(c) thermophile
(d) hyper-thermophile
(a) psychrophile: 10-30 C
(b) meophile: 20-40 C
(c) thermophile: Greater than 45 C
(d) hyper-thermophile: 80-121 C
Ch. 9
Describe the following..
- obligate aerobe
- obligate anaerobe
- falculative anaerobe
- aerotolerant anaerobe
- microaerophile
- capnophile
obligate aerobe:
cannot grow w/o O2
obligate anaerobe:
lack actablic enzyme systems
falculative anaerobe:
uses O2 whenever present
aerotolerant anaerobe:
grown on limited O2 , not harmed
microaerophile:
Harmed by O2, but require small amount of it for metabolism
capnophile:
grows best @ higher CO2
Ch. 19
Which of the following is the type of cell largely responsible for type I hypersensitivity responses?
mast cell
Ch. 19
Type I hypersensitivities require which of the following initial priming events to occur?
sensitization