Studied by 7 people
• Glass
• Plastic
Red top tubes are made of two materials:
Glass
– No additive
– Glass surface activates clotting sequence
– Do not mix
– SERUM: use for TDM (Therapeutic Drug Monitoring)
Plastic
– Contain additive to activate clotting sequence
– Do invert to mix additive and initiate clotting sequence
– SERUM
60 minutes
What is the blood clotting time of red top tube?
RED-TOP TUBE
Laboratory use: Serum determinations in chemistry, routine blood donor screening, and diagnostic testing for infectious diseases
GOLD OR MOTTLED-RED-GRAY TOP TUBE
• Contain clot activator and gel (SST)
• Invert to mix and initiate clotting sequence
• SERUM
30 minutes
What is the blood clotting time of gold or mottled red-gray top tube?
GOLD OR MOTTLED-RED-GRAY TOP TUBE
Laboratory use: serum determination in chemistry, blood donor screening, serum testing for infectious diseases
LIGHT BLUE-TOP TUBE
• Anticoagulant – 3.2 % sodium citrate
LIGHT BLUE-TOP TUBE
• Specimen- Plasma
• Binds calcium
LIGHT BLUE-TOP TUBE
3-4 full gentle inversion
LIGHT BLUE-TOP TUBE
Blood: anticoagulant ratio critical (9:1)
LIGHT BLUE-TOP TUBE
• Laboratory use: Clot-based studies/coagulation studies
GREEN-TOP TUBE
• Anticoagulant = heparin
GREEN-TOP TUBE
• Inhibits thrombin formation
GREEN-TOP TUBE
• Must be full and on ice if needed for pH, ionized Ca analysis
GREEN-TOP TUBE
• Laboratory use: plasma determinations in chemistry
Lithium heparin
Ammonium heparin
Sodium heparin
What are three formulations of green-top tube?
PURPLE-TOP TUBE
• Anticoagulant: Liquid K2EDTA (glass) or spray-coated K3EDTA (plastic)
PURPLE-TOP TUBE
• Sample: Can either be plasma or whole blood
• Binds calcium
PURPLE-TOP TUBE
Requires 8 full inversions
PURPLE-TOP TUBE
• Laboratory use: Hematology determinations (CBC), routine immunohematology testing, and blood donor screening
GRAY-TOP TUBE
• Anticoagulant = potassium oxalate
– Binds calcium
– PLASMA, Whole blood
• Antiglycolytic agent = sodium fluoride
– Maintains plasma glucose levels
GRAY-TOP TUBE
• Laboratory use: glucose determinations and lactic acid level determination
potassium oxalate
What is the anticoagulant of gray-top tube?
potassium oxalate
It is the anticoagulant of gray-top tube that:
– Binds calcium
– PLASMA, Whole blood
sodium fluoride
What is the antiglycolytic agent of gray-top tube?
sodium fluoride
– Maintains plasma glucose levels
• ACD = acid citrate dextrose
• SPS = sodium polyanethol sulfonate
what are the two anticoagulants of yellow-top tube?
– Blood bank studies
– HLA phenotyping
– Paternity testing
– DNA
What are the test performed in ACD = acid citrate dextrose?
– Used for special blood culture studies
– Inhibits certain antibiotics
What are the test performed in SPS = sodium polyanethol sulfonate
YELLOW-TOP TUBE
• ACD = acid citrate dextrose
• SPS = sodium polyanethol sulfonate
• Both bind calcium
• PLASMA, Whole blood
• Blood Culture or sterile tubes
• Coagulation tube
• Serum tube
• Heparin Tube
• EDTA tube
• Oxalate/fluoride tube
ORDER OF DRAW
yellow stopper
What is color of the stopper of blood culture or sterile tubes
light blue stopper
What is color of the stopper of coagulation tube?
red, gold, or red gray marbled
What is color of the stopper of serum tube with or without clot activator or gel ?
green or light green stopper
What is color of the stopper of heparin tube?
lavender stopper
What is color of the stopper of EDTA tube?
gray stopper
What is color of the stopper of oxalate/fluoride tube?
Rotate between palm and mix
Inversion of Blood Gases tube?
10 Inversions
Inversion of Slides & Smears tube?
Invert 10 times
Inversion of Lithium heparin tube?
10 inversions
Inversion of Lithium heparin with gel separator tube?
10 times
Inversion of Sodium fluoride/ Potassium oxalate tube?
5 Inversions
Inversions of Serum-Clot activator tube?
0 Inversions
Inversions of Serum-No Additive tube?
Recommended to be collected separately
Inversions for Newborn Blood Spot Card?
Note 
Flashcard (33)