MLS blood bank final

RBC CPD, ACD-A, and CP2D shelf life

21 days

RBC CDPA-1 shelf life

35 days

RBC CPD-AS (additive solution)

42 days

RBC response

increase the hemoglobin by 1 mg/dL or hematocrit by 3%

shelf life of frozen and deglycerolized RBC

10 years at -65 degrees c or 24 hours if deglycerolized in open system

indications for plasms transfusion

coagulopathy

storage of frozem plasma

-18c for 1 year or -65c for 7 years

storage for thawed FFP

1-6c for 24 hours or can be thawed and held for additional 4 days at 1-6c

liquid plasma storage

kept at 1-6c for 26-40 days

indications for cryo

hemophilia A if factor 8 concentrate not avaliable, hypofibrinogenemia, VWB disease, fibrin glue

what is in cryo

sources of fibrinogen, factor 8, factor 13, and vWF

shelf life of cryo

1 year at -18c, Store at room temp after thawing and only viable for 4-5 hours after thaw

shelf life of platelets

5 days in closed system, 7 days if additional bacterial detection testing

platelet transfusion response

raise the platelet count 5,000-10,000

QC for irradiated RBC

RDP must have <803×10^5 WBC

SDP must have <5.0 ×10^6 WBC

indications for washed products

reduced exposure to plasma proteins. IgA deficiency, plasma incompatible platelets

indications for Leukocyte reduced components

hematopoietic cell transplant, chronically transfused patients, and patients wit multiple febrile transfusion reactions

indications for CMV neg products

intrauterine transfusions

infants less than 4 months

cogenital immunodificiency

BM transplant recipients

CMV neg patients

irradiated products do what

inactivate T-lymphocytes and prevents proliferation; graft vs host disease. it reduces the storage to 28 days or RBC expiration due to release of potassium

washed products storage

RBC: 24 hours open system at 1-6c

just known granulocytes are for…..

severe neutropenia

storage and expiration of granulocytes

room temp with no agitation; expires in 24 hours

cold stored platelet information

stored at 1-6c with no agitation

has a 14 day shelf life

only used for treatment of active bleeding when conventional are not available

whole blood transfusion goals

increase oxygen capacity and volume expansion

packed RBC transfusion goals

increase RBC mass and oxygen carrying capacity

platelet QC

greater than or equal to 5.5×10^10 PLT and a pH greater than 6.2

a reason to transfuse a patient with deglycerolized RBCs

an antibody to a high incidence antigen

which would be most beneficial to transfuse platlets

functional platelet abnormalities

minimum hemoglobin concentration required for a male blood donor

13.0 g/dL

positive test for T. cruzi will cause what

indefinite deferral of blood donation

in order to be a platlet pheresis donor, the platelet count must be at least

150,000/uL

A donor who just donated 2 units of apheresis red cells will be deferred from further blood donation for a minimum of how many weeks

16

all donor blood testing must include

serological syphilis testing

transport temperature of platelet

20-24c

hematocrit of whole blood

40%

packed RBC hematocrit

less than or equal to 80%

CPDA-1 = 65-80%

AS = 55-80% the hematocrit must be within specific ranges for various preservation solutions.

frozen deglycerolized RBC

80% recover with <1% glycerol

free of WBC, platelets, and plasma due to wash step

reasons to use frozen deglycerolized RBC

rare phenotypes and autologous use

frozen deglycerolized RBC storage

-65c good for 10 years

once deglycerolized 1-6c good for 24 hours

single donor platlets QC

greater then or equal to 3×10^11 and pH greater than 6.2

FFP must be frozen within how many hours of collection

8

QC for cryoprecipitate

80 IU AHF

dosing interval for whole blood

8 weeks

dosing interval for RBC (double red)

16 weeks

dosing interval for platelet

7 days (no more than 24 times a year)

dosing interval for plasma

4 weeks (28 days)

height and weight reqirement for donation

110 pounds (double red males 130 and females 150)

temperature requirments

less than or equal to 37.5 or 99.5f

pulse requirements for donation

50-100 BPM

blood pressure

upper: 180/100

lower 90/50

hemoglobin requirements for allogenic donation

males: >13 g/dL

females: >12.5 g/dL

hematocrit requirements for allogenic donation

males: >39%

females: >38%

hemoglobin and hematocrit requirements for autologous donation

hgb: >11 g/dL, hct > 33%

blood transfusion length of deferral

3 months

chronic fatigue syndrome length of deferal

indefinite

cancer of blood length of deferral

temporarily deferred

heart disease length of deferral

6 months

history of chagas disease length of deferral

indefinite

hepatitis B length of deferral

permanant

needle stick length of deferral

3 months

smallpox vaccine length of deferral

8 weeks (or 14-21 days after scab falls off)

pregnancy length of deferral

6 weeks after birth

1 unit of single donor platlets increases PLT count by

30,000-60,000

1 unit of random donor platlet increases PLT count by

5,000-10,000