phlebotomy (copy)

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1

yellow

Additive Specimen Type: SPS (Sodium Polyanetholsulfonate) ACD (acid citrate dextrose)

Specimen type: whole blood

Test Types: Plasma Cultures, HIV Cultures, Blood culture, Specialty blood banking (paternity/DNA)

Inversions: 8-10

Special instructions: Sterile (aseptic technique)—clean site with iodine rather than 70% alcohol swab

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2

light blue

additive: Sodium Citrate (anticoagulant

specimen type: plasma or whole

test types: Coagulation studies: PT—Prothrombin time; time it takes for a clot to form. PTT— partial thromboplastin time; time for # of blood to clot

inversions: 3-4

special instructions: Ask if the patient is taking blood thinners—this will affect the times of coagulation studies. Use a trash tube if light blue is the ONLY tube! Fill all the way

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3

red

additive: none

specimen type: serum

test types: Drug screen Blood chemistries,AIDS, antibody tests, viral studies, serological tests, blood grouping and blood typing

inversions: 0

special instructions: N/A

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4

gold/tiger

additive: Clot activator + SST—serum separating tube using thixotropic gel to separate serum from buffy coat and hematocrit

specimen type: serum

test types:

  • Serum determination: same as tiger top

  • Lipid Panel (cholesterol + triglycerides) Liver enzyme, Pregnancy test, CMP (14 tests), BMP( 8 tests). Endocrine and serological tests, including HIV and hepatitis

inversions: 5

special instructions: N/A

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5

green

additive: Sodium or Lithium Heparin (anticoagulant)

specimen type: plasma or whole

test types: Electrolyte Studies, arterial blood gas tests; Chemistry: Non-fasting glucose; BUN; ionized Ca2+; Creatinine

inversions: 8

special instructions: n/a

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6

lavender

additive: EDTA (ethylenediaminetetraacetic acid)

specimen type: whole

test types: Hematology: CBC (w/ or w/o differential), H&H, ESR (erythrocyte sedimentation rate), Sickle cell anemia

inversions: 8

special instructions: at least 2/3 full

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7

pink

additive: EDTA

specimen type: whole

test types: Blood banking: ABO-blood test determination, antibody identification.

inversions: 8

special instructions: n/a

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8

navy

additive: EDTA

specimen type: plasma or whole

test types: Nutritional Studies: Heavy metal (arsenic, lead, mercury), toxicology screens, TDM (therapeutic drug monitoring)

inversions: 8

special instructions: n/a

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9

grey

additive: Potassium oxalate Sodium Fluoride

specimen type: plasma or whole

test types: Fasting blood glucose (FBG), GTT (glucose tolerance test), blood alcohol levels, Lacti acid

inversions: 8

special instructions: Glucose is FASTING 8+ hours, otherwise reschedule the patient appointment.

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10

Tube Labels should include:

Pt Name
Pt ID#
Date/Time
MA Initials

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11

BC (Blood Culture)

yellow

microbiology

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12

CBC

lavender

hematology

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13

CMP (complete metabolic panel)

gold

chemistry

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14

CMV (complete metabolic volume)

Gold
Blood Bank

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15

drug screening

red

chemistry

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16

FBG (fasting blood glucose)

grey

chemistry

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17

HCT (hematocrit)

lavender

hematology

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18

hep b. antigen

red

serology

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19

hep c antibody

red

serology

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20

hep c antibody + RNA (viral load)

gold

serology

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21

HGB (Hemoglobin)

Lavender
Hematology

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22

HIV - CD4

Lavender
Hematology

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23

HIV - EIA - Westernbl

red

serology

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24

HIV - Genotype

Gray, Plasma Prep
Serology

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25

HIV - RNA (Viral Load)

Gray, Plasma Prep
Serology

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26

PT/INR

Light Blue
Coagulation

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27

RBC (Red Blood Cells)

Lavender
Hematology

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28

Syphilis Titer

Red
Serology

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29

Syphilis - Antibody

Red
Serology

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30

WBC (White Blood Cells)

Lavender
Hematology

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31

Cephalic Vein

The cephalic is the second vein of choice

This is the vein used for most obese patients.

<p><span>The cephalic is the second vein of choice </span></p><p><span>This is the vein used for most obese patients.</span></p>
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32

brachial vein

<p></p>
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33

basilic vein

The Basilic vein is the third vein of choice due to the location of the nerves by the vein

<p>The Basilic vein is the third vein of choice due to the location of the nerves by the vein</p>
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34

median cubital vein

Median cubital is the first vein of choice.

<p><span>Median cubital is the first vein of choice.</span></p>
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35

sclerosed veins

Sclerosed veins are damaged veins and are not suitable for venipuncture

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36

capillaries

Are microscopic one cell- thick vessels that link arterioles and venules that form a bridge between arteries and veins.
1. Only vessels that permit the exchange of CO2 and O2 between blood and other tissues
2. Blood in the capillary bed is a mix of arterial and venous blood

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37

coagulation issues that affect phlebotomy

Drugs like Heparin and Coumadin suppress clotting and may result in patient bleeding more than normal

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38

Parts of a multi-sample needle:

1.) Bevel
2.) Shaft
3.) Threaded hub
4.) Rubber sleeve over needle

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39

Needle size

1. The smaller the number of the needle the bigger it is.
a. most butterfly needles are usually a 23 to 25

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40

Glucose Tolerance Test

(GTT) used to diagnose carbohydrate metabolism problems. Patient must eat well balanced meals 3 days prior to test and must fast at least 12 hours before the test. Patients must drink glucose prior to testing and have 5 minutes to finish the drink. Levels will peak within 30 minutes to an hour following glucose ingestion.

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41

Lactose Tolerance Test

Same procedures as GTT but patient must drink lactose rather than glucose

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42

Anticoagulants

Prevent blood from clotting.

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43

Hematoma

When blood seeps into surrounding tissue during a venipuncture.

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44

Clotting Disorder Patients

Need pressure to be applied for at least 5 minutes to insure blood stoppage.

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45

Specimen Labels

patients name, date and time of collection draw, collectors initials.

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46

Syncope

Patient might faint during venipuncture

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47

Unlabeled Tubes

must be rejected automatically

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48

Post-Prandial

after meal

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49

Chain of Custody

MUST be followed EXACTLY when drawing patient test samples that may be used in legal proceeding.

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50

Coumadin is known as what type of medication?

anti-coagulant

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51

What color tube do you use for glucose testing?

grey

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52

What is the most common analytical error that occurs during blood draw?

wrong order of draw

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53

what is the purpose of EDTA in a lavender tube?

to prevent platelets from clumping together and maintain the appearance of the cells for microscopic examination

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54

cbc and pt on patient requires what tubes?

light blue and lavender

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55

which tubes and order of draw Pt, electrolytes, and blood culture

yellow, light blue, green

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56

2 liters =

2000 mls

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57

what tube(s) are needed for h & h, esr, reticulocyte ?

lavender - 1 tube

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