Central Dogma: DNA to RNA to Protein in Eukaryotes

RNA polymerase is positioned and activated to start transcription of a gene.

2

the methionine is transferred to the second amino acid by formation of a peptide bond.

9

The small subunit of the ribosome binds to the mRNA, and along with initiation factors, and methionine tRNA, finds the start codon.

6

An aminoacyl tRNA enters the A site on the ribosome next to the start codon.

8

The portions of the pre-mRNA sequence transcribed (introns) are removed by RNA splicing.

4

Processed RNA exits the nucleus.

5

An enhancer binding protein binds to the correct site on DNA and recruits RNA polymerase to the promoter

1

The newly made protein is folded

12

The newly transcribed pre-mRNA is made

3

When the A site has a stop codon, a termination factor, rather than a charged tRNA, binds, and allows the hydrolysis of the completed peptide from the final tRNA.

11

The complete ribosome is assembled by the addition of the large subunit

7

The ribosome shifts along the mRNA, so that the A site in now aligned with the third codon in the coding region of the message

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