Cell Culture and A549 Cell Line Flashcards

Flashcards about cell culture and A549 cell line.

Carbon Source

Energy source for cells in culture, often glucose, pyruvate, or glutamine.

Buffering System

Maintains stable pH (7.2-7.4) in cell culture using a CO2-bicarbonate system.

Phenol Red

pH indicator in cell culture media, turns yellow in acidic conditions and pink in basic conditions.

Serum

Essential component of cell culture media, a mixture of sugars, salts, lipids, and growth factors like FBS.

Metabolites, Vitamins, and Minerals

Amino acids, vitamins, and inorganic salts provided by growth media like DMEM.

Antibiotics

Prevent unwanted bacterial or fungal growth in cell cultures.

A549 Cell Line

Cancerous alveolar basal epithelial cells.

A549 Cells Characteristics

Squamous cells responsible for diffusion of water and electrolytes across alveoli, grow as a monolayer in vitro.

A549 Cells Applications

Studying lung cancer metabolism and drug delivery mechanisms.

Peptide Drug

KAPI (KRAS and PDE delta Inhibitor)