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Eukaryotic Cells
Cells with a nucleus and complex structures.
Prokaryotic Cells
Cells without a nucleus, mostly bacteria.
Organelles
Subcellular structures with specific functions.
Cytoplasm
Jelly-like substance where chemical reactions occur.
Ribosomes
Sites for protein synthesis from mRNA.
Mitochondria
Powerhouse of the cell, site of respiration.
Cell Membrane
Selectively permeable barrier surrounding the cell.
Cell Wall
Rigid structure providing support in plants.
Chloroplasts
Organelles responsible for photosynthesis in plants.
Permanent Vacuole
Storage for cell sap, maintains rigidity.
Flagellum
Tail-like structure aiding in cell movement.
Pili
Hair-like structures for attachment to surfaces.
Slime Capsule
Protective layer preventing dehydration and toxins.
Plasmids
Circular DNA rings providing extra genetic information.
Micrometres
Unit of measurement; 1,000 µm = 1 mm.
Nanometres
Unit of measurement; 1,000 nm = 1 µm.
Scientific Notation
Method to express very small or large numbers.
Magnification
Size of image divided by actual size.
Resolution
Smallest distance between two distinguishable points.
Light Microscopes
Use light to magnify small objects.
Electron Microscopes
Use electrons for higher magnification and resolution.
Cellular Measurement Units
Measurements for cells in micrometres and nanometres.
Nucleus
Contains genetic material, controls cell functions.
Cellular Structures
Components within a cell performing specific roles.
Cell Sap
Liquid found in vacuoles, maintains turgor pressure.
Chlorophyll
Green pigment in chloroplasts for photosynthesis.
Aerobic Respiration
Process of producing energy using oxygen.
Resolution
Ability to distinguish two close points.
Light Microscope
Uses light to view samples with 0.2µm resolution.
Transmission Electron Microscope (TEM)
Uses electrons, achieving 0.1nm resolution.
Scanning Electron Microscope (SEM)
Uses electrons, achieving 1nm resolution, produces 3D images.
Sample Preparation
Process of preparing specimens for microscopy.
Eukaryotic Cells
Cells with a nucleus, viewed by light microscopes.
Iodine Stain
Stain used to enhance visibility of samples.
Double Helix
Structure of DNA formed by two strands.
Polymer
Material made of repeating units, like DNA.
Nucleotide
Monomer of DNA, consisting of a base, sugar, phosphate.
Chromosome
Long DNA molecule, humans have 46 chromosomes.
Adenine (A)
Base that pairs with Thymine in DNA.
Thymine (T)
Base that pairs with Adenine in DNA.
Guanine (G)
Base that pairs with Cytosine in DNA.
Cytosine (C)
Base that pairs with Guanine in DNA.
Complementary Base Pairing
Specific pairing of DNA bases (A-T, G-C).
Transcription
Process of copying DNA to mRNA.
Messenger RNA (mRNA)
Single-stranded RNA copied from DNA.
Uracil (U)
Base used in RNA, replaces Thymine from DNA.
Electron Beam
Stream of electrons used in electron microscopy.
Black and White Images
Colorless images produced by electron microscopes.
3D Imaging
Technique used by SEM to visualize surface structures.
Stain
Substance used to enhance visibility of biological samples.
Living Samples
Specimens that can be viewed using light microscopes.
Dead Samples
Specimens required for SEM and TEM imaging.
Resolution Power
Measurement of a microscope's ability to resolve details.
Nucleus
Cell organelle containing DNA.
Cell Thickness
Ideal sample thickness for microscopy, preferably 1 cell thick.
mRNA
Single strand of genetic information from DNA.
Translation
Process of coding amino acids from mRNA.
Ribosomes
Cellular structures that synthesize proteins.
Codons
Triplet nucleotide sequences in mRNA.
Amino Acids
Building blocks of proteins coded by mRNA.
Proteins
Molecules formed by amino acid chains.
Enzymes
Proteins that speed up chemical reactions.
Biological Catalysts
Substances that accelerate chemical reactions.
Active Site
Region on enzyme where substrates bind.
Lock and Key Hypothesis
Enzyme specificity based on active site shape.
Lipase
Enzyme that digests lipids in the gut.
Enzyme Reaction Steps
Substrate binds, reaction occurs, products released.
Optimum Temperature
Temperature at which enzyme activity is highest.
Denature
Permanent alteration of enzyme shape.
pH Level
Measure of acidity affecting enzyme activity.
Substrate Concentration
Amount of substrate influencing reaction rate.
Enzyme Concentration
Amount of enzyme affecting reaction speed.
Metabolic Rate
Rate of energy transfer from food.
Carbohydrates
Energy-containing biomolecules, often polymers.
Sugars
Simple carbohydrates like glucose and sucrose.
Starch
Polysaccharide formed from glucose in plants.
Respiration
Process of energy release from food.
Cytoplasm
Cellular fluid where ribosomes are located.
Substrate-Enzyme Complex
Temporary structure formed during enzyme reaction.
Chemical Bond
Attraction between atoms in molecules.
Reaction Rate
Speed at which reactants convert to products.
Moderated Diet
Balanced intake of carbohydrates, proteins, lipids.
Chemical Stores
Energy reserves within food molecules.
Carbohydrase
Enzyme that breaks down carbohydrates.
Amylase
Enzyme that breaks down starch.
Benedict's Reagent
Tests for sugars; color indicates concentration.
Proteins
Polymers made of amino acids.
Amino Acids
Building blocks of proteins.
Protease
Enzyme that breaks down proteins.
Biuret's Solution
Tests for proteins; turns purple if present.
Lipids
Fats made of fatty acids and glycerol.
Lipase
Enzyme that breaks down lipids.
Sudan III
Tests for lipids; red layer indicates presence.
Aerobic Respiration
Uses glucose and oxygen to produce energy.
Respiration Equation
C6H12O6 + 6O2 -> 6CO2 + 6H2O.
Mitochondria
Cell organelles where aerobic respiration occurs.
ATP
Energy currency produced during respiration.
Anaerobic Respiration
Energy production without oxygen, creates lactic acid.
Lactic Acid
Waste product of anaerobic respiration.
Oxygen Debt
Deficit of oxygen during strenuous exercise.