Lab practical study - types of media

TSA

Grow bacteria (NOT selective or differential)

TSA - Carbon source

tryptone

TSA - Energy Source

Glucose

Phenylethyl Alcohol Agar (PEA) - Selective or differential?

Selective (only gram positive)

MacConkey Agar - Selective or differential?

Selective + Differential

MacConkey Agar - Selective

Selects gram -

MacConkey Agar - Differential

Selects lactose fermentation

How to know if MacConkey Agar is a lactose fermenter?

Pink colonies are fermenters, colorless are not

Eosin Methylene Blue (EMB) - Selective or differential?

Selective + Differential

Eosin Methylene Blue (EMB) - Selective

Only Gram -

Eosin Methylene Blue (EMB) - Differential

Identifies lactose fermenter and E.coli

How to know if Eosin Methylene Blue (EMB) is a lactose fermenter?

Pink colonies are fermenters, colorless are not

How to know if Eosin Methylene Blue (EMB) grows E.Coli

Metallic Green

Mannitol Salt Agar (MSA) - Selective or differential?

Selective + Differential

Mannitol Salt Agar (MSA) - Selective

Selects halophiles

Mannitol Salt Agar (MSA) - Differential

tests Mannitol fermentation

Foy you have growth on a MSA plate that is yellow what does that mean?

You have a halophile and it ferments mannitol

Blood Agar

Detect hemolysis

Starch Agar tests for

Amylase

Casein (Milk) Agar tests for

caseinase

Tributyrin Agar tests for

Lipase

Gelatin Tube tests for

gelatinase

how to know if Gelatin Tube tests positive

It is liquid

SIM Agar is apart of what tests?

IMViC

SIM Agar tests what