investiagte the effect on temperature on the action of an enzyme

What’s an enzyme

Enzymes are biological catalyst that speed up reactions in cells without being used ip

Word equation of hydrogen peroxide being broken down

Safety

You must wear eye protection when carrying out this practical assessment.

Care must be taken when working with hydrogen peroxide.

Apparatus and materials

• 1 x boiling tube rack containing four boiling tubes labelled 1, 2, 3 and 4

• Timer

• 5 cm3 syringe

• 10 cm° syringe

• Waterproof marker pen

• Ruler

• 1 x water bath at 20°C

• 1 x water bath at 30°C

• 1 small beaker of hydrogen peroxide

• 1 small beaker of catalase enzyme solution

• 2 thermometers one for each water bath

• Safety glasses

Method

1. Label each of boiling tubes 1 to 4 with your initials using the marker pen.

2. Use the 5 cm3 syringe to add 5 cm° of catalase enzyme solution into each of boiling tubes 1 and 3.

3. Use the 10 cm° syringe to add 10 cm§ of hydrogen peroxide into each of boiling tubes 2 and 4.

4, Place boiling tubes:

• 1 and 2 into the 20°C water bath.

• 3 and 4 into the 30°C water bath.

5, Leave the boiling tubes in the water baths for at least five minutes

* 6. Remove boiling tubes 1 and 2 from the 20°C water bath and place them in the boiling tube rack on your bench.

1. Carefully pour the contents of boiling tube 2 (hydrogen peroxide) into boiling tube 1 (catalase enzyme solution). Return boiling tube 1 to the water bath and start the timer immediately. Leave for three minutes.

2. After three minutes, remove boiling tube 1 from the water bath. Hold the ruler against the outside of boiling tube 1 and measure the height of froth in mm above the level of the liquid.

Record this height in the table for 20°C.

1. Remove boiling tubes 3 and 4 from the 30°C water bath and place them in the boiling tube rack on your bench.

2. Carefully pour the contents of boiling tube 4 (hydrogen peroxide) into boiling tube 3 (catalase enzyme solution). Return boiling tube 3 to the water bath and start the timer immediately. Leave for three minutes.
   11, After three minutes, remove boiling tube 3 from the water bath. Hold the ruler against the outside of boiling tube 3 and measure the height of froth in mm above the level of the liquid.

Record this height in the column in the table for 30°C.