06. Gene Therapy

Can gene therapy be used for chromosomal disorders?

No

Down syndrome

extra copy of chromosome 21 (triplet instead of doublet)

List important Genome editing techniques

1. CRISPER Cas-9

2. Finger zinc nucleases

3. Talens

4. Site directed nuclease

5. Homing endonuclease/ MegaNucleases

What is Bacterial immune system aginst viruses

CRISPER Cas-9

How does crisper-cas9 works in bacteria

when a bacteria sees a virus DNA, the guide RNA of bacteria goes there and first unfolds the virus DNA , then the guide RNA attaches to the virus DNA then cas-9 protein comes and cuts at the point where guide RNA is present, so virus DNA splits into parts hence becomes inactive

1. Function of CRISPR

2. Function of cas-9

1. to create guide RNA

2. to cut

What is the useof CRISPR-Cas9 when we already have restriction endonuclease

RE are not very specific, they can cut at multiple places where as CRISPR-Cas9 is very specific because of guide RNA hence we can modify guide RNA to cut at desired points precisely

is CRISPR-Cas9 is used only for cutting or pasting too?

It only cuts, pasting is not its duty