DNA polymerase slides

DNA polymerase parts

polymerase

3’→ 5’ exonuclease

polymerase what does it do, parts

adds nucleotides to the 3’ end of nucleic acids

left: fingers

middle: palm

right: thumb, longer

3’→ 5’ exonuclease

removes nucleotides from the 3’-end of DNA

DNA polymerase adds, type of bond formed

dNTPs to the 3’-end of a DNA strand

dNTP selection

phosphodiester-bond formation

DNA polymerase can only add dNTPs to a nucleic acid strand (“primer”), it cannot link 2 dNTPs together.

DNA polymerase is

highly processive

adds many nucleotides without dissociating from the template

3’ end of product strand remains in polymerase active site

polymerase moves 3’→5’ on the template strand

DNA polymerase is 1

highly processive

DNA polymerase adds

adds many nucleotides without dissociating from the template

DNA polymerase active site

3’ end of product strand remains in polymerase active site

DNA polymerase how does it move

polymerase moves 3’→5’ on the template strand