GTP Protein Detection and Techniques and Application part 2

What are fluorescently labeled antibodies used for in research?

They detect proteins with high sensitivity and specificity using primary and secondary antibodies.

What is the Lifecore system?

A labeling system commonly used in teaching labs for fluorescent antibody detection.

What are alternative methods to fluorescent labeling for protein detection?

Chemiluminescence and colorimetric detection.

How does chemiluminescence work in protein detection?

A substrate reacts to produce light, which is captured on x-ray film.

What is the principle of colorimetric detection in protein assays?

An enzyme-substrate reaction produces a visible color change, often used in histology.

What is the difference between immunohistochemistry (IHC) and immunocytochemistry (ICC)?

IHC detects proteins in tissues; ICC detects proteins in cells.

How do IHC and ICC relate to western blotting?

They are variations of immunoblotting that allow for in situ protein detection.

What is dual antibody detection used for?

To detect total protein and post-translational modifications like phosphorylation simultaneously.

Why are loading controls important in western blotting?

They ensure equal protein loading across samples and validate experimental results.

What are future topics that build on protein detection?

Microscopy, protein localization, and purification techniques.