Cytochrome Oxidase

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5 Terms

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How does test work

The dye replaces oxidase as electron transfer mechanism in aerobic respiration.

2
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Positive test

If oxidase is there, dye will turn purple

3
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Negative test

If no oxidase, colorless

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Dye name

Tetra-methyl-p-phenylenediamine dihydrochloride (Do not memorize just recognize)

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Caveats

  1. Interpret quickly

  2. Do not grab colony with metal (false positive)

  3. Stay away from MacConkey/ pigmented colonies