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genome
entire set of DNA instructions found in cell
gene
unit of heredity transferred parent to offspring
genotype
genetic characteristics
phenotype
physical characteristics
antiparallel
strands run in opposite directions
semiconservative
during DNA replication, 2 strands of nucleotides separate
helicase
unwinds DNA
ligase
“glue” DNA fragments together
DNA polymerase I
removing RNA primers
DNA polymerase III
add nucleotides to the 3’ end of growing
RNA primer
provides 3’ end onto which polymerase can add nucleotides
SSB proteins
binds to single stranded regions of DNA (in e. coli)
replication fork
where the parent DNA strands split
leading strand
continuously synthesized strand
lagging strand
replicates discontinuously forming short fragments
okazaki fragments
short lwngths of DNA on lagging strand
topoisomerase IV
bacterial enzyme unlinks and resolves DNA knots
transcription
DNA→RNA
translation
mRNA → amino acids
polyribosome complex
mRNAs with multiple ribosomes attached
mRNA
carries the genetic info to make proteins
tRNA
decodes mRNA sequence into protein
rRNA
links amino acids together
promoter
where RNA polymerase begins transcriptions
exons
region of genome that ends up in mRNA
introns
noncoding sections that get spliced out
repressible operons
transcription of gene is repressedi
inducible operons
gene can be turned on by presence
phase variation
reversible switch on/off expressing phase
vertical gene transfer
parent to offspring (binary fission)
horizontal gene transfer
acquire DNA not from parent cell
transformation
foreign genetic material is taken up by cell
transduction
virus transfers genetic material from one to another
bacteriophage
virus that infects bacteria
mutations
change in DNA sequencemutag
mutagens
chemical/physical agent that induces mutations